FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005408443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44151610
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG1367730.309780322846664No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1150060.2604797424148293Illumina Single End PCR Primer 1 (100% over 32bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1135430.2571661599656275No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT1049050.23760175450000579Illumina Single End PCR Primer 1 (96% over 33bp)
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA670920.15195821851117094No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG618000.1399722456327187No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC606260.13731322595031076No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT605060.1370414351820919No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT587020.13295551396653485No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA579670.13129079551119427No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG579650.13128626566505727Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGC579130.13116848966549577Illumina Single End PCR Primer 1 (97% over 35bp)
CCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGG551930.12500789891920136No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA540850.12249836415931378No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG496750.11251005342727026Illumina Single End PCR Primer 1 (100% over 32bp)
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT482930.10937992974661627No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG478710.10842413221171324Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG474000.10735735344645417Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG464830.10528041899264828Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)