FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6917401
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGC794911.14914546662829No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA423140.6117037309243747No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG379690.5488911225473266No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC330870.4783154829393294No Hit
AGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACG327030.4727642650758573No Hit
GGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGG234500.33900015338130607No Hit
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG217790.3148436818972906No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189450.2738745375611447No Hit
GCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGG168050.24293806300950313No Hit
CCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCA161480.2334402761962188No Hit
CGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGG151420.21889724189764334No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT140390.2029519468366804No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138800.2006533956900865No Hit
GGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAG121380.17547052715318948No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG119410.1726226367388561No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG119190.17230459821542804No Hit
GGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGCG101770.147121729678531No Hit
CAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAC100190.14483763482845652No Hit
CTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTC89200.12895016495357142No Hit
CCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTC87360.12629020639399105No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA82320.11900423294818387No Hit
GCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAAC77960.1127012876656999No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG76830.11106772615900105No Hit
CGGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCG75310.10887036908804333No Hit
GGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGC75150.10863906834373199No Hit
CGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGGCGGC75090.10855233056461525No Hit
GGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGC74040.10703441943007207No Hit
CACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCACGGGCCCCTCTC70700.10220601639257286No Hit
CGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAA69490.10045680451371837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)