FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43239808
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1064985224.629739336492893No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT692038216.004654784776104No Hit
GCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGC1043240.24126841636299584No Hit
CGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGCC1028120.23777163857896874No Hit
GGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCG983210.22738537599426897No Hit
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG919520.21265589338417043No Hit
CGGGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCC862370.19943890592668678No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTAT767130.17741290618126704TruSeq Adapter, Index 23 (97% over 37bp)
CCCAGACCCGCCGCGACGCCGCCGAGAACCGCCCCCGCGCCCGCCGACAC747210.17280604021183443No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG450900.10427890891652432No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTAT434080.10038897490016606TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)