FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383660
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31173022.52937860457049No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160714.570559241432143No Hit
GCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGC49410.35709639651359437No Hit
CGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGCC47250.3414856250813061No Hit
GGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCG42280.3055663963690502No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCTCGTAT42040.30383186620990704TruSeq Adapter, Index 23 (97% over 37bp)
CGGGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCC36790.2658890189786508No Hit
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG33530.24232831765028984No Hit
CCCAGACCCGCCGCGACGCCGCCGAGAACCGCCCCCGCGCCCGCCGACAC24810.17930705520142232No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCACAACAATCGCGTAT20840.15061503548559618TruSeq Adapter, Index 23 (97% over 37bp)
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG15760.113900813783733No Hit
GCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGGCCCCGCG15310.11064856973533961No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA14140.10219273520951679No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)