FastQCFastQC Report
Tue 12 Jul 2022
EGAF00005408521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11695374
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3286232.809854563009272TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC765050.6541475287579517TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGG387880.3316524978166581TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC336770.28795145841424136TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC259790.22213056204957618TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC200880.17176021904045136TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGC191200.16348344225674186TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGG173140.14804143929044083TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC156370.13370243653601843TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTTTGC151290.1293588388024188TruSeq Adapter, Index 2 (97% over 46bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)