FastQCFastQC Report
Tue 12 Jul 2022
EGAF00005408589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30243203
Sequences flagged as poor quality0
Sequence length86
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT603140.19942993471954673No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC558210.18457370404847662TruSeq Adapter, Index 4 (100% over 50bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT373760.12358479358155286No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG349020.11540444310743143No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT321730.10638092797247697No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA309260.1022576874545993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)