FastQCFastQC Report
Thu 7 Jul 2022
EGAF00005408623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38768174
Sequences flagged as poor quality0
Sequence length86
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT2712950.6997879239811501No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT2165560.5585922101979836No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA1949080.502752592887145No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA1769000.45630212039390866No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC1755720.45287663019671753No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC1590450.41024630151525837No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCA1519230.39187556267158724No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT1515230.3908437885158068No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT1511060.3897681639584057No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG1348260.3477749558181409No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG1312050.3384348202729383No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG1188770.3066355407917845No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA1105300.28510499359603575No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA1103780.2847129194168392No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA1048810.2705337630810262No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTG1004310.2590552755979686No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG962540.2482809739762311No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA962350.24823196470383155No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT922070.2378419989551223No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA879700.22691293121001777No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA872090.22494998087864548No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC843320.2175289452631945No Hit
GCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTT822200.21208117772067367No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC805270.20771419360633286No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAG778860.2009019047427924No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT760910.19627181821872758No Hit
GCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCA757950.19550830534345No Hit
GCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCG749770.19339832719487896No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC702670.18124918651056407No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGG676100.17439562668079234No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG675350.1742021690265835No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCG675110.1741402625772367No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG639580.16497552863851675No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG623790.16090260015857336No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCG616190.1589422292625905No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATG603070.15555801003163058No Hit
GGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGG590850.15240593998572127No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG584190.1506880360163468No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA572060.14755918088894254No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG566670.1461688652140284No Hit
GGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGG541690.13972543561117942No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG532800.13743231754995733No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA519050.13388559388946203No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG513570.13247206329604277No Hit
GCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTATGAC511830.13202324153827827No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTG510600.1317059709853758No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT504100.13002933798223254No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG489630.12629689497369673No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCA468030.12072531453248224No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA464370.11978124117994311No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT453840.11706509571485105No Hit
GCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCC452180.11663690944020216No Hit
CTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAA435690.11238342048299722No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC430740.1111065999652189No Hit
GTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTATG424150.10940675204357059No Hit
CCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAAC422160.1088934444010698No Hit
AGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAA421370.10868966900530316No Hit
CCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAA415550.10718843760864258No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC414180.10683505496028778No Hit
GGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTT411440.10612828966357817No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG411300.10609217756812586No Hit
GCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCAC401590.10358754580496879No Hit
CCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAG401350.10352563935562195No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT394220.1016865019229433No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG392960.10136149306387245No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAG390460.10071663421650964No Hit
GCCGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAGT390280.10067020437949953No Hit
GGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCA390010.10060055962398434No Hit
CGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGG388220.1001388406892726No Hit
GTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTC387910.10005887819219961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)