FastQCFastQC Report
Tue 12 Jul 2022
EGAF00005408626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20132929
Sequences flagged as poor quality0
Sequence length86
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2155641.070703621912142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG996310.49486589854859164No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT987050.49026646843089744No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA903720.44887656435881734No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT863730.4290135826734401No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC746300.37068625235801506No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG721200.35821911456599287No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG666080.3308410812952254No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT641000.3183838774775394No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCG634230.3150212271647111No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC605820.30091001662003575No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGC600320.2981781736775608No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCA560340.2783201589793517No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTG537000.2667272109289215No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA536930.2666924420187445No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA495590.2461589170656689No Hit
TGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGT461820.22938540139887248No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT459470.22821815941436044No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATT452040.22452768794843514No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA432440.21479239309888787No Hit
GAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGT431030.2140920479081807No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA422540.2098750758024329No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC422240.20972606618738882No Hit
CCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAAC396750.1970652158958093No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA396590.19698574410111913No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT380920.1892024752086495No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA379320.18840775726174766No Hit
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG347920.17281141755379956No Hit
TTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTG347720.17271207781043682No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAG335400.16659274961929285No Hit
TTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGC333930.1658626025055768No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGG318070.15798496085691258No Hit
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA318010.15795515893390377No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA317870.15788562111354984No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCA316480.15719520989717892No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC315580.15674818105204663No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGG309440.15369845093081091No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA306270.1521239159985117No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG296590.14731587241975572No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG292310.14519000191179338No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT285030.1415740352533901No Hit
GCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCG284900.14150946442020432No Hit
GCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCA282650.14039189230737364No Hit
CCAGAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAGT278580.13837032852994216No Hit
CTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAG275500.13684049648215618No Hit
GTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGAT272680.13543980610074172No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG271700.13495304135826436No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC270370.1342924320649022No Hit
CGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGA268070.13315002501623086No Hit
TGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCT267780.13300598238835493No Hit
GAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGG265720.13198278303171881No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG263760.1310092535467641No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGC262800.13053242277862304No Hit
CGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCG260770.12952412438349134No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG260620.1294496195759693No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA260510.1293949827171198No Hit
GCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTT255420.12686678624853842No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTG240130.11927226286845795No Hit
CCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGA238020.11822422857598117No Hit
CAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACG237080.11775733178217636No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATG236710.11757355325695532No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC232670.11556689044102822No Hit
GGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTT224610.11156349878351034No Hit
TGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGA222310.11042109173483898No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG221720.11012803949191895No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT218910.10873231609767262No Hit
CAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGT217040.10780348949723113No Hit
GGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGG216060.10731672475475376No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAG215780.10717764911404594No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG211190.10489780200387136No Hit
TCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGG209630.10412295200564209No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG208920.10377029591670442No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA208610.10361631931449221No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG207070.1028514032905992No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205640.1021411241255557No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT205520.10208152027953805No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC204930.10178846803661802No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCC203650.10115269367909657No Hit
ACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGG202910.10078513662865449No Hit
GGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAA202270.10046724944989376No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT202170.1004175795782124No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG201990.10032817380918593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)