FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037434
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT28987427.941440130167315TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT263612.540980920232034No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG244742.3590898312567354No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA228232.1999471773626085No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG198641.9147242137813103No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA192281.8534191090710348No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT189771.829224798878772No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG112211.0816109747704432No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA111671.0764058243705141No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT93380.9001054524914356No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC74480.7179251884939186TruSeq Adapter, Index 27 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA71620.6903571697091092No Hit
GAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGT59780.576229427606961TruSeq Adapter, Index 27 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC54760.5278408072224353No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC49640.47848827009718214No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC48690.46933106106026984No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT38440.37052959513569056No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC35970.3467208516397188No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA32910.3172249993734541No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC31680.3053688234625046No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG31100.2997781063662845TruSeq Adapter, Index 27 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA29110.2805961632258052No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC28460.2743307044110758No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA27440.26449875365565423No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT25970.2503291775669585TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC25350.24435289377444736No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC20140.19413283158253922No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGACCTCGTAT18340.17678233024944237TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGGACCCCCCAGCCTGCGACTCT17060.16444419596812906No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGTCTGGTCCTACGCTGGTGAA14210.13697256885739237No Hit
AGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCTC11700.11277825866512954No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAACTCGTAT11280.10872980835407361TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCATCTCGTATG11270.10863341668000084TruSeq Adapter, Index 27 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT274300.0280.021451
GTCCAGC294300.0268.65682
TGTCCAG295500.0268.23821
TCCAGCA301850.0262.03443
AGCAGTG300100.0255.433232
AGCACGC312750.0252.807986
CCAGCAC313550.0252.162984
GTGGTAT304750.0247.002676
CACGCTT322350.0245.370228
ACGCTTC322750.0245.202739
CAGCACG325900.0242.832695
GATCGGA397300.0241.421631
TGGTATC312350.0240.851557
TCGGAAG401400.0238.237053
ATCGGAA402800.0237.91982
AGTGGTA322250.0233.862695
GGTATCA323550.0232.605098
CGGAAGA411500.0232.032524
GTATCAA324850.0231.131359
CAGTGGT328150.0230.240134
Produced by FastQC (version 0.11.5)