FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037434
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT29035627.987900917070384Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT252322.432154720203888No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG250202.4117196853004628No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA234052.256047131672955No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG225892.177391525629582No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT189851.8299959322713542No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA182351.7577021767167837No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA117061.1283609366957321No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG107361.0348610128451545No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT92110.887863709884195No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA72020.6942128366720196No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT57090.5503000672813886No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC51440.49583877143027894No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC48380.4663429191640143No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC43790.4220991407646173No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC37330.35983011931361414No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA32680.31500799086978065No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC31440.3030554232847584No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA30860.29746470618853826No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA30680.2957296560552286No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCGGTG30580.294765739314501Illumina Single End PCR Primer 1 (96% over 29bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC30320.2922595557886092No Hit
AAGCAGTGGTATCAACGCAGAGTCTCAGGGACCCCCCAGCCTGCGACTCT27590.2659446287667456No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC26870.2590044282335069No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC23150.22314672547844006Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC19050.18362613910860834No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCGATAGTGTAGATCT17650.17013130473842192Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTGTCTGGTCCTACGCTGGTGAA16410.15817873715339964No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCCATAGTGTAGATCT13240.12762257647233463Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGGGCCTCAGTGAAGGTCTCCTG13050.12579113466495218No Hit
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT10690.10304269958378075Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATATAGTGTAGATCT10470.10092208275418002Illumina Single End PCR Primer 1 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT295250.0281.332341
TGTCCAG281650.0273.020841
AGCAGTG316150.0262.19432
GTGGTAT313150.0257.526556
GTCCAGC300900.0256.094852
TCCAGCA305600.0252.252383
TGGTATC319600.0251.455697
AGCACGC313750.0245.465656
AGTGGTA330800.0244.718935
GGTATCA328300.0244.31158
CAGTGGT333450.0243.479224
GTATCAA328450.0243.215629
ACGCTTC315950.0242.791479
CACGCTT317150.0242.289818
GATCGGA394450.0241.961531
CCAGCAC319400.0241.07754
TCGGAAG398500.0239.429353
ATCGGAA398900.0239.373442
CGGAAGA402200.0237.044054
GAGCGTC399750.0236.596779
Produced by FastQC (version 0.11.5)