FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281221
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT6542023.2628431020443TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA79132.8138012452839583No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT77002.7380601021972044No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT66942.3803343278062448No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA57162.0325651356050933No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA49111.746313397648113No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT45931.6332350713495793No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA41931.4909981829237504No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG29031.0322842177504525No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC23480.834930535059615No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG23270.8274630984172591No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG21530.7655900519520236No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC18830.669580152264589No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC15090.5365886615864391No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC12970.4612031107207499No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA12300.4373784319094236No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC11660.41462052976129093No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC11440.40679750089787037No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA11000.39115144317102923No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA9310.33105635781111653No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT7630.27131686467226845No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA6120.21762243929151803No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC5810.20659908043851632No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC5590.19877605157509576No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCCGT4980.17708492609015686TruSeq Adapter, Index 6 (96% over 31bp)
AGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCTC4620.16428360613183227No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAGCTCGTAT4610.1639280139107677TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCTC4440.15788294615266996No Hit
AGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCAC3820.13583622844666648No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAACCTCGTAT3400.12090135516195448TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG3160.11236714185640474TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG87500.0286.693941
AAGCAGT79450.0282.262851
GTCCAGC91950.0272.659332
TCCAGCA93850.0268.078863
AGCACGC97400.0257.855386
GCACGCT98200.0255.904377
CAGCACG98600.0255.462395
CACGCTT98850.0254.518978
ACGCTTC99100.0253.72869
GATCGGA84700.0248.978331
CCAGCAC101400.0248.553134
ATCGGAA85050.0247.780962
TCGGAAG84950.0247.553653
CGGAAGA85350.0246.221274
GTGGTAT90050.0242.998756
AGCAGTG92400.0241.908052
GGAAGAG86650.0241.679265
AGAGCAC87150.0237.931928
TGGTATC93000.0235.132697
GAAGAGC89200.0233.452266
Produced by FastQC (version 0.11.5)