FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281221
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT6335522.528545165545957Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA81512.8984321938973263No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT74042.632804804762091No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT68612.439718228724028No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA59532.116840491997397No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT48801.7352900387951113No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA46961.66986107011923No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA41501.4757077174179738No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG28671.0194828977921278No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG25380.9024930570618838No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG25340.9010706881776255No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC23460.8342193506174859No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC20250.7200742476557583No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC14060.49996266281678825No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA13300.4729376540158808No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC12230.43488928636197155No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT11950.42493270417216356No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC11680.4153317142034201No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA11340.40324157868722466No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC10400.36981590990715485No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA9440.33567905668495596No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC5940.21122177931235578No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC5870.20873263376490375No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA5320.1891750616063523No Hit
AAGCAGTGGTATCAACGCAGAGTCTCAGGGACCCCCCAGCCTGCGACTCT3240.11521187962492133No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTCGGTG3000.1066776663193716Illumina Single End PCR Primer 1 (96% over 29bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG81650.0285.390351
AAGCAGT86800.0282.569061
GTCCAGC88800.0263.27722
TCCAGCA90500.0259.14353
GTGGTAT92800.0252.404086
AGCACGC92950.0251.680596
GCACGCT92600.0251.044977
CACGCTT92750.0250.005238
GATCGGA84000.0249.823681
CAGCACG93550.0249.595155
ACGCTTC92850.0249.419459
AGCAGTG98350.0247.872332
ATCGGAA84550.0247.834612
TCGGAAG84550.0247.487013
CGGAAGA84550.0246.791824
GAGCGTC84400.0246.359919
TGGTATC94850.0246.174227
AGAGCGT85400.0243.991328
AAGAGCG85500.0243.534097
CCAGCAC97050.0241.502244
Produced by FastQC (version 0.11.5)