FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237841
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT147766.212553764910171TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT94373.9677767920585603No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT81503.4266589864657484No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT79893.3589667046472225No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA74403.128140228135603No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG68172.866200528924786No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA50142.1081310623483756No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA44321.8634297703087357No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT38721.6279783552877762No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG26121.0982126714906175No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC19010.7992734642050782No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC18460.7761487716583768No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG18370.7723647310598256No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA16300.6853317972931496No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC13700.5760150688905613No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA11000.46249385093402734No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA10750.4519826270491631No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC8380.3523362246206499No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA7350.30902998221500916No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG6160.25899655652305537No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC6020.2531102711475314No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC5530.23250827233319737No Hit
AGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCTC4700.19761100903544804No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC4570.19214517261531863No Hit
AGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCTC4260.17911125499808694No Hit
AGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTC4240.1782703570872978No Hit
AGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCAC3360.14127084901257564No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC3230.13580501259244623No Hit
AGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTGC2740.11520301377811228No Hit
AGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCCTG2490.10469178989324801No Hit
AAGGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTC2480.10427134093785344No Hit
GTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCCTGT2460.10343044302706429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG98700.0285.462041
AAGCAGT85300.0285.162481
GTCCAGC102550.0275.031682
TCCAGCA102950.0274.24863
CCAGCAC105250.0268.395174
CAGCACG105600.0267.644785
AGCACGC105800.0267.277746
CACGCTT107150.0263.361638
GCACGCT107300.0263.26747
ACGCTTC107250.0263.116069
AGCAGTG94000.0258.300722
GTGGTAT98200.0240.667796
TGGTATC99550.0236.66597
GCAGTGG103050.0234.6183
AGTGGTA102900.0229.675195
GGTATCA102700.0229.40698
GTATCAA103400.0227.853879
CAGTGGT110800.0214.360614
AAGCAGG1400.0199.466031
GGGTATC2450.0161.972438
Produced by FastQC (version 0.11.5)