FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237841
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT109654.610222795901463Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT94433.9702994857909277No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT91393.842483003350978No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT80673.3917617231679986No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA77773.2698315261035735No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG71853.0209257445099875No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA60622.5487615676018853No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA50792.1354602444490225No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT36681.542206768387284No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG32781.3782316757834014No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG20490.8614999096034746No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC18420.7744669758367986No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC18360.7719442821044311No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA15730.6613662068356592No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC15340.6449686975752709No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA10980.46165295302323817No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC10800.45408487182613594No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA9930.4175058127068083No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA6810.28632573862370236No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG6110.25689431174608246No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC5780.24301949621806165No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC5500.23124692546701367No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATAT5360.22536064009148968Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC4960.20854268187570688No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC3140.1320209719938951No Hit
AGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCTC3120.13118007408310595No Hit
AGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCTC3070.1290778293061331No Hit
AGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCAC2710.11394166691192857No Hit
AAGCAGTGGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTC2700.11352121795653398No Hit
AGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTC2640.11099852422416658No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATTT2430.10216909616088059Illumina Single End PCR Primer 1 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG85100.0287.641
AAGCAGT93050.0286.69131
GTCCAGC94500.0259.54852
AGCAGTG102950.0258.22972
TCCAGCA95500.0257.754033
GTGGTAT101800.0253.06276
AGCACGC97550.0251.282826
CCAGCAC97800.0250.640464
CAGCACG98050.0250.001425
TGGTATC102650.0249.965047
CACGCTT98300.0249.36568
GCACGCT98550.0248.882147
ACGCTTC98550.0248.58399
GGTATCA105000.0244.790478
GTATCAA105700.0242.474189
GCAGTGG109400.0242.199043
AGTGGTA108200.0238.229935
CAGTGGT117650.0221.21814
AAGCAGG1650.0178.845721
GATCGGA29700.0153.509231
Produced by FastQC (version 0.11.5)