FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397214
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT9379823.613971310175373Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT131983.3226422029435017No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT108972.7433574848822047No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA88182.219962035577799No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA85322.147960545197299No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT82802.0845186725543408No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT77321.9465577749021938No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA50551.2726137548021974No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA40671.0238813334877421No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG34330.864269638028871No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG31170.7847155437623046No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG25740.6480134134245017No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCGCCTGTGTAGATCT24480.6162924771030225Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC21420.5392559174651448No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC17780.44761765698087175No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG17320.4360369976889032No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCGCCTGTGTAGATCT16420.4133791860307039Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA16310.4106098979391462No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGTCTGGTCCTACGCTGGTGAA16020.4033090475159486No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC14390.3622732330683209No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC13340.3358391194670883No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC13220.3328180779126617No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC11710.29480330501946056No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTACGCCTGTGTAGATCT10610.2671104241038836Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC10140.2552780113490461No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC9380.2361447481710111No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGTCGCCTGTGTAGATCT8700.2190255126959271Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA8020.20190627722084317No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTCGCCTGTGTAGATCT7380.18579405559723475Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCCGTGTAGATCT6600.16615728549346198Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGCTTCGCCTGTGTAGATCT6250.15734591429305111Illumina Single End PCR Primer 1 (96% over 31bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA6170.15533188659010005No Hit
AGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCTC4220.1062399613306681No Hit
AGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCTC4160.1047294405534548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG102000.0286.137881
AAGCAGT120150.0282.45761
GTCCAGC110950.0261.43352
TCCAGCA113000.0257.210853
GTGGTAT129250.0256.932656
AGCAGTG133400.0253.786212
TGGTATC133350.0248.041247
CAGCACG117500.0247.610325
AGCACGC118050.0246.581166
GCACGCT118450.0245.128227
GGTATCA135450.0243.870188
CCAGCAC119250.0243.606954
ACGCTTC119600.0243.5399
CACGCTT119400.0243.054788
GTATCAA136550.0241.290369
AGTGGTA139150.0238.652855
GATCGGA151300.0238.250471
GCAGTGG141250.0238.017553
TCGGAAG150750.0237.931463
ATCGGAA151350.0237.570722
Produced by FastQC (version 0.11.5)