FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357742
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT11734632.8018516137328TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA75882.12108167338473No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA74882.0931285675151368No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT71111.9877453583867704No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA71051.9860681720345947No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT56131.5690078324602648No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT51401.436789641697089No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA41121.149431713357671No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC40981.1455182785359281No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG37051.035662572468427No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG27000.7547338584790156No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA26770.7483046441290091No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC23540.6580161121702233No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC22250.621956605598448No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG18970.5302704183461825No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT13270.3709377148895014No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC11660.3259332144394564No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC11260.3147519720916191No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC8940.24990076647416296No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCCGT8590.24011717941980534TruSeq Adapter, Index 6 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAGCTCGTAT8480.23704233777415007TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA6770.18924252673714576No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA6620.1850495608567068No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAACCTCGTAT5300.1481514611088438TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG4970.13892693617187807TruSeq Adapter, Index 6 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA4970.13892693617187807No Hit
AGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCAC3630.10146977430662323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG97450.0284.279021
GTCCAGC100300.0276.347842
AAGCAGT89300.0275.82771
TCCAGCA101450.0273.07043
GATCGGA142750.0263.662751
CAGCACG105650.0262.07575
ATCGGAA143950.0261.668982
TCGGAAG143850.0261.54443
CCAGCAC106300.0260.749664
CGGAAGA144450.0260.254554
AGCACGC106950.0258.75276
CACGCTT107950.0256.491858
GGAAGAG146350.0256.47415
GCACGCT108050.0256.118447
ACGCTTC108150.0255.745769
AGAGCAC146850.0254.29988
AGCAGTG98200.0250.380082
GAAGAGC151600.0247.30146
AAGAGCA152450.0245.536937
GAGCACA152950.0243.965569
Produced by FastQC (version 0.11.5)