FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393950
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT17712444.96103566442442TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT152513.871303464906714Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT102492.601599187714177Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA98972.5122477471760374Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA46351.1765452468587383Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA37250.9455514659220713Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC19700.5000634598299277Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG17540.445234166772433Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA16780.4259423784744257Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC15820.40157380378220586Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC15640.3970046960274146Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC14880.3777129077294073Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC13090.33227566950120574Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG12570.31907602487625336Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAGCTCGTAT12350.3134915598426196TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG10920.2771925371240005TruSeq Adapter, Index 6 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC10470.2657697677370225Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCTC10210.25916994542454624Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT8080.20510217032618352Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAACCTCGTAT8020.20357913440791978TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT7960.20205609848965603Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGGTCTGGTCCTACGCTGGTGAA7470.1896179718238355Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCTC7180.18225663155222743Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTGTCTGGTCCTACGCTGGTGAA6770.1718492194440919Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCAC6530.16575707577103693Clontech Universal Primer Mix Long (95% over 21bp)
GAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCCGT5550.14088082243939587TruSeq Adapter, Index 6 (96% over 31bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC5200.13199644624952406Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA5110.12971189237212843Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA5110.12971189237212843Clontech Universal Primer Mix Long (100% over 23bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAACTCGTAT4960.1259043025764691TruSeq Adapter, Index 6 (97% over 36bp)
AGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCTCCTG4310.10940474679527858No Hit
AGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCTCCTG4160.10559715699961923No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAAATCTCGTATG4060.103058763802513TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)