FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393950
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT13537034.36222870922706Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT161384.096458941490036No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA106452.7021195583195836No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT97102.464779794390151No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATAT52811.340525447391801Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA47081.195075517197614No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA36740.9326056606168296No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATTT28560.7249650970935397Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC21980.5579388247239497No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG20780.527478106358675No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCAGTACGTGTAGATCT20230.5135169437745907Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCAGTACGTGTAGATCT19760.5015864957481914Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA17420.44218809493590555No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC14660.37212844269577355No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC14610.37085924609722043No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC14430.36629013834242924No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC12910.32770656174641455No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCA12150.3084147734484071Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG12090.3068917375301434No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATGT10430.26475441045818Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC10350.262723695900495No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT9930.2520624444726488No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGAGCT9390.23835512120827518Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGGAGATCT8500.21576342175402968Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGAACT8450.21449422515547656Illumina Single End PCR Primer 1 (96% over 32bp)
AGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCTC8160.2071328848838685No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT7650.19418707957862671No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGTCTGGTCCTACGCTGGTGAA7440.18885645386470365No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACAGTACGTGTAGATCT7420.1883487752252824Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGCTCT7420.1883487752252824Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTCGATCT6020.15281127046579515Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGCACGTGTAGATCT5800.14722680543216143Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTTTCACCAGCGTAGGACCAGACACCTAGGCTCTGCGTTGAT5450.13834242924228962No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC5380.13656555400431528No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA5320.13504251808605153No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCG5260.13351948216778778Illumina Single End PCR Primer 1 (96% over 32bp)
AGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCTC4930.12514278461733724No Hit
AGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCAC4810.12209671278080976No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTTGATCT4690.11905064094428228Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA4620.11727376570630792No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTCCGTGTAGATCT4570.1160045691077548Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGTCTCAGGGACCCCCCAGCCTGCGACTCT4420.11219697931209544No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCC4290.10889706815585734Illumina Single End PCR Primer 1 (96% over 32bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT4240.10762787155730422Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGACCT4230.10737403223759359Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG64350.0274.635561
AAGCAGT83300.0269.005551
GATCGGA213650.0268.040921
ATCGGAA216200.0264.619172
TCGGAAG215950.0264.585333
GAGCGTC214850.0262.657629
CGGAAGA218050.0261.835024
AGAGCGT217150.0260.619878
AAGAGCG218300.0259.919927
GGAAGAG220750.0258.299745
GAAGAGC221550.0256.37236
GTGGTAT89950.0241.899486
TGGTATC91000.0238.462527
GGTATCA92850.0234.34428
AGCAGTG96200.0232.445222
GTATCAA93750.0231.467629
GCAGTGG100500.0221.330293
AGTGGTA99200.0219.639545
CAGTGGT101200.0218.057224
GTCCAGC82700.0213.00742
Produced by FastQC (version 0.11.5)