FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485378
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT21820844.9563020985706TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT96111.9801062264873974No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT88631.8259995302630116No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT82751.7048568332310075No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA68311.4073567405197598No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA53931.1110927977782263No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA52031.0719480487372728No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG47560.9798548759935556No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA45750.9425643519071735No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG43040.8867315782750764No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG38180.7866034307282159No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA36340.7486948316569766No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT23560.4853948881078252No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA22740.46850083852172947No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC18060.37208114088401206No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGT17610.3628100161111546TruSeq Adapter, Index 2 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC16780.3457099415301064TruSeq Adapter, Index 2 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA16230.3343785668077251No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC15250.3141881173023911No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC13810.2845205180292473No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG12440.2562950937207702TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAGCTCGTAT10600.2183864946495309TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTACCTCGTAT8810.1815080205530535TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG8730.1798598205934344No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT7350.15142837129000492TruSeq Adapter, Index 14 (97% over 38bp)
AATGATACGGCGACCACCGAGATCTACACTATAGCCTACACTCTTTCCCT6920.14256929650705222Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA6580.13556444667867107No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC6220.1281475468603851No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGCATTATCTCGTAT6170.12711742188562317TruSeq Adapter, Index 11 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC6030.12423307195628974No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATCATCTCGTAT4960.10218839749638427TruSeq Adapter, Index 2 (97% over 37bp)
AGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCTC4930.1015703225115271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG97450.0279.890841
GATCGGA260900.0274.876921
TCGGAAG262050.0273.838873
ATCGGAA262300.0273.689942
AAGCAGT97050.0273.321781
CGGAAGA266350.0268.97664
GGAAGAG268450.0266.325045
GTCCAGC102900.0264.781042
AGAGCAC273000.0261.347968
GAAGAGC273350.0260.852026
TCCAGCA105550.0258.690223
AAGAGCA278200.0256.146037
GAGCACA278700.0255.89749
AGCAGTG105600.0249.800372
CCAGCAC110200.0247.774524
AGCACGC110650.0246.766856
CACGCTT110800.0246.034888
CAGCACG111200.0245.810645
ACGCTTC110850.0245.791349
GCACGCT111600.0244.929617
Produced by FastQC (version 0.11.5)