FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485378
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT21738444.78653750272983Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT95811.973925476638826No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT90671.8680286292332986No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT86711.7864427312321531No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA70211.4465014895607136No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA54911.1312832472835603No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA54391.1205699475460362No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG50001.030124974761938No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG48771.0047839003827945No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA42600.8776664784971713No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG39140.8063818302436452No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA34740.7157308324645946No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA22200.4573754887943005No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC20390.4200849647079184No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT20010.41225601489972763No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC15990.32943396692886784No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC15750.3244893670500105No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA13950.2874048679585807No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCGATAGTGTAGATCT13790.28410846803934253Illumina Single End PCR Primer 1 (97% over 34bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCGGTG11790.24290346904886503Illumina Single End PCR Primer 1 (96% over 29bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT8280.17058869582057695Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG8130.16749832089629113No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCCATAGTGTAGATCT7980.1644079459720053Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATATAGTGTAGATCT7750.15966937108810042Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC7260.14957414633543342Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC6240.1285595968502899No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC5570.1147559221884799No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC5310.10939927231971784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG80100.0280.065521
GATCGGA258350.0276.825871
TCGGAAG259300.0275.582123
ATCGGAA260350.0274.92222
AAGCAGT106450.0273.920141
GAGCGTC259500.0272.50949
CGGAAGA262650.0271.787454
AGAGCGT262900.0270.27118
GGAAGAG264350.0270.150825
AAGAGCG264600.0269.284677
GAAGAGC267950.0266.027686
GTGGTAT111400.0251.808296
AGCAGTG117650.0247.299122
TGGTATC117950.0237.07747
GGTATCA120100.0233.394828
GTATCAA120250.0231.881329
GCAGTGG124600.0231.500293
AGTGGTA122000.0230.893435
GTCCAGC97450.0229.801282
TCCAGCA99100.0226.568223
Produced by FastQC (version 0.11.5)