FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413825
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT21859052.82184498278257TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT115682.7953845224430616No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA53501.29281701202199No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG51311.2398960913429589No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT50441.2188727118951248No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA47141.1391288588171329No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT46631.1268048087959888No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA40230.9721500634326105No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG28450.6874886727481423No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG27790.6715399021325439No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA17520.42336736543224796No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC12960.31317585936084097No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC12020.29046094363559477No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAGCTCGTAT11900.28756116716003144TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC10410.25155560925511994No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGTCTGGTCCTACGCTGGTGAA9810.23705672687730323No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGT9460.22859904549024346TruSeq Adapter, Index 2 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG9220.2227994925391168TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTACCTCGTAT9030.2182081797861415TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC8800.21265027487464508TruSeq Adapter, Index 2 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA8240.1991179846553495No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC8160.19718480033830724No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA7150.17277834833564912No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT7000.16915362774119494No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGCATTATCTCGTAT6690.16166253851265633TruSeq Adapter, Index 11 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC6290.15199661692744518No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT6150.14861354437262128TruSeq Adapter, Index 14 (97% over 38bp)
AGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCTC5950.14378058358001572No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATCATCTCGTAT5050.12203226001329065TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA265500.0273.742221
TCGGAAG266250.0272.62923
TGTCCAG60650.0272.38021
ATCGGAA266850.0272.126342
CGGAAGA267750.0270.607974
GGAAGAG270950.0267.140845
AAGCAGT71650.0265.640081
GAAGAGC273850.0263.775366
AGAGCAC275400.0261.863958
AAGAGCA278650.0259.020637
GTCCAGC63700.0258.814422
GAGCACA282600.0255.036249
TCCAGCA65850.0250.587313
AGCAGTG78250.0242.612532
AGCACGC68750.0239.375896
GTGGTAT77550.0238.360296
CACGCTT69250.0237.435358
ACGCTTC69200.0237.394589
GCACGCT69750.0235.943957
CAGCACG70450.0234.22535
Produced by FastQC (version 0.11.5)