FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355607
Sequences flagged as poor quality0
Sequence length300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT24248868.18988377619112TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT34190.9614546395318427No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT31240.8784978923362027No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT24760.696274257818322No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA23760.6681533265655625No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA18410.5177063443632999No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA16960.47693099404679884No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGT15140.4257508991667768TruSeq Adapter, Index 2 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC13870.3900373164757724TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAGCTCGTAT11890.33435787259530886TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA11710.3292961049698122No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTACCTCGTAT10640.29920670852935966TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG10110.28430261496539716No Hit
AATGATACGGCGACCACCGAGATCTACACCCTATCCTACACTCTTTCCCT9030.2539320092124171Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG8350.23480977596054073No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA8050.22637349658471292No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG7320.20584521677019857TruSeq Adapter, Index 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG7300.20528279814514336No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT6980.19628410014426037TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGCATTATCTCGTAT6650.1870041928308498TruSeq Adapter, Index 11 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC6530.18362968108051866No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA6290.1768806575798564No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATCATCTCGTAT6070.17069405270424937TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA5820.16366381989105952No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTT5130.14426037732665556No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCATTATCTCGTAT4920.13835498176357608TruSeq Adapter, Index 11 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC4770.13413684207566218No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC4500.12654419063741715No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAACTCGTAT4450.1251381440747792TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA4040.11360856226114785TruSeq Adapter, Index 2 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCACTATCTCGTAT4010.11276493432356505TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATATCTCGTATG3790.10657832944795799TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGGTCTGGTCCTACGCTGGTGAA3620.10179777113498892No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCACATTATCTCGTAT3600.10123535250993372TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA287900.0275.229221
TCGGAAG289050.0273.841433
ATCGGAA289300.0273.70642
CGGAAGA291850.0270.811344
GGAAGAG293900.0268.72245
GAAGAGC298650.0264.153146
AGAGCAC299800.0262.453588
GAGCACA303550.0259.21139
AAGAGCA303950.0259.208627
TGTCCAG25750.0250.013661
AAGCAGT39450.0234.725561
GTCCAGC27400.0234.38872
AGCAGTG39800.0231.520922
GTGGTAT39800.0229.674676
GCAGTGG40850.0225.21023
AGCACGC29250.0220.568976
GCACGCT29400.0219.94357
TCCAGCA29550.0217.832373
TGGTATC42000.0217.644077
AGTGGTA42200.0216.264335
Produced by FastQC (version 0.11.5)