FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355607
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT24300068.33386294420526Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT30670.8624689615221297No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT28080.7896357495774831No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT20490.5761978813690394No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA18810.5289547168644037No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA15970.449091272106567No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA13780.3875064326630241No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA10280.28908317327836625No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTCGGTG9980.2806468939025385Illumina Single End PCR Primer 1 (96% over 29bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG9160.2575877302752758No Hit
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT7960.22384261277196454Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG7740.2176560078963575No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAGAGGGTGTAGATCT6600.1855981462682118Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA6080.17097526201677696No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG5820.16366381989105952No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCCGAAGATCTT5360.1507281915147902No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATAT5180.14566642388929352Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC5050.1420107028264348No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATATGGTGTAGATCT4820.13554288863830014Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTCTCAGGGACCCCCCAGCCTGCGACTCT4140.11642065538642378No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTAGGGTGTAGATCT4020.11304614363609265Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTCAGGGACCCCCCAGTCT3970.11164009707345468No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC3810.10714074807301319No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC3800.10685953876048558No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA3730.10489107357279244No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATTT3610.10151656182246131Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA285500.0277.374761
TCGGAAG284850.0276.71933
ATCGGAA286750.0275.398252
CGGAAGA286550.0274.462254
GAGCGTC286650.0272.815649
AGAGCGT288100.0272.054788
AAGAGCG288850.0271.869847
GGAAGAG290900.0270.358065
GAAGAGC290900.0270.20656
TGTCCAG26100.0252.136541
AAGCAGT35900.0234.272811
GTGGTAT34400.0232.384616
TGGTATC35100.0227.331517
GCAGTGG37900.0221.392963
GGTATCA37150.0214.817188
CAGTGGT38850.0213.331514
AGCAGTG39550.0212.89972
GTCCAGC31200.0211.003832
AGTGGTA38250.0210.5315
GTATCAA39800.0199.775489
Produced by FastQC (version 0.11.5)