FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179194
Sequences flagged as poor quality0
Sequence length300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT9541953.24899271180955TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT27861.55473955601192Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT26721.4911213545096376Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA21671.2093038829425091Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT19241.073696663950802Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT18221.0167751152382336Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA16630.9280444657745237Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA15880.8861903858388115Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA15150.8454524147013851Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG13840.7723472884136746Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG12530.699242162125964Clontech Universal Primer Mix Long (95% over 22bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC10190.5686574327265422TruSeq Adapter, Index 2 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG8110.45258211770483386Clontech Universal Primer Mix Long (95% over 22bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGT7180.4006830585845508TruSeq Adapter, Index 2 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA7080.39510251459312257Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA5940.3314843130908401Clontech Universal Primer Mix Long (95% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG4790.26730805718941486TruSeq Adapter, Index 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC4600.2567050236057011Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAGCTCGTAT4570.2550308604082726TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG4340.24219560922798755Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC3790.21150261727513198Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTACCTCGTAT3590.2003415292922754TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC3000.1674163197428485Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGCATTATCTCGTAT2870.16016161255399175TruSeq Adapter, Index 11 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA2840.15848744935656328Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT2560.1428619261805641TruSeq Adapter, Index 14 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC2290.1277944574037077Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA2270.12667834860542207Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATCATCTCGTAT2250.1255622398071364TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC2200.12277196781142224Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTCATTATCTCGTAT2070.11551726062256548TruSeq Adapter, Index 11 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCACATTATCTCGTAT1820.10156590064399476TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)