FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368244
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT14099238.28765709692487TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT109532.9743865480496625No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT67041.820532038539664No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT59331.611159991744604No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG56311.5291491511063318No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA45421.2334213184736207No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA42431.1522251550602318No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG31610.8583982359522491No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA31480.8548679679777538No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA29610.8040864209600157No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA26190.7112132173232966No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG26120.7093123037985684No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC20810.5651144349941887No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC16760.455133009634916No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC13740.3731221689966435No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC11380.3090342273058081No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT9920.2693866023614777No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA8380.227566504817458No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC7820.21235919661963262No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA7630.20719957419537047No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTAGCTCGTAT7100.1928069432224286TruSeq Adapter, Index 2 (97% over 37bp)
AGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCTC7020.19063447062273928No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG6220.1689097446258459TruSeq Adapter, Index 2 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC5590.15180152290329238No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTACCTCGTAT5290.14365475065445737TruSeq Adapter, Index 2 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC5160.14012448267996222No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGCATTATCTCGTAT4600.12491717448213685TruSeq Adapter, Index 11 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC4540.12328782003236984No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCATTATCTCGTAT4200.11405481148369016TruSeq Adapter, Index 14 (97% over 38bp)
AGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCTC4160.11296857518384548No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC3950.10726583460966099TruSeq Adapter, Index 2 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCCGT3920.10645115738477748TruSeq Adapter, Index 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG89650.0283.43421
AAGCAGT79800.0274.772641
GTCCAGC94950.0267.148932
TCCAGCA96150.0263.50913
GATCGGA178250.0259.545071
TCGGAAG179050.0258.221253
ATCGGAA179000.0258.211272
CGGAAGA180500.0255.576974
AGCACGC98850.0255.419536
CAGCACG99450.0254.026295
CCAGCAC99800.0253.577214
GCACGCT100200.0251.831577
CACGCTT100400.0251.329918
ACGCTTC100350.0251.162259
GGAAGAG183850.0250.999945
GAAGAGC184600.0249.741356
AGAGCAC184850.0249.006048
AGCAGTG88900.0246.481032
AAGAGCA188100.0244.93817
GAGCACA191700.0240.031669
Produced by FastQC (version 0.11.5)