FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368244
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT12463733.84630842593498Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT109952.985792029198032No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT66861.815643975190363No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT65931.7903889812189746No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG58971.6013838650460022No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA48061.3051129142633688No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA46971.2755129750926015No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA30940.8402037779298509No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG30810.8366735099553556No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG29220.7934956170365302No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCGCCTGTGTAGATCT28730.7801892223634329Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA28020.7609085280411901No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGTCTGGTCCTACGCTGGTGAA25780.7000792952498887No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCGGGGAGTCTCTGAAGATCTC20820.5653859940691498No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCGCCTGTGTAGATCT17910.48636230325545016Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC16500.4480724736859256No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC14690.3989202811179544No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTACGCCTGTGTAGATCT12950.35166900207471136Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT11610.315280086029915No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGTCGCCTGTGTAGATCT9340.25363617601373Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC9320.2530930578638077No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC8310.2256655912927298No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTCGCCTGTGTAGATCT8020.21779037811885596Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA7750.21045828309490447No Hit
AAGCAGTGGTATCAACGCAGAGTCTCAGGGACCCCCCAGCCTGCGACTCT6980.1895482343228946No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA6540.17759963502460324No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCCGTGTAGATCT6440.17488404427499157Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC6280.1705390990756129No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGCTTCGCCTGTGTAGATCT6080.16510791757638957Illumina Single End PCR Primer 1 (96% over 31bp)
AGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCTC5560.15098684567840887No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGCGCCTGTGTAGATCT5560.15098684567840887Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC4990.13550797840562234No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC4990.13550797840562234No Hit
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT4690.12736120615678737Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCGCCTGTGTAGATCT3730.10129153496051531Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG75200.0280.962551
AAGCAGT91550.0274.1381
GATCGGA176700.0260.770661
TCGGAAG176450.0260.242833
ATCGGAA177350.0259.336432
CGGAAGA177600.0258.061284
AGAGCGT178050.0256.17118
GAGCGTC177450.0256.126379
AAGAGCG179100.0255.653817
GGAAGAG180400.0254.218785
GAAGAGC181400.0252.736366
AGCAGTG101000.0247.767122
GTCCAGC85550.0247.68272
GTGGTAT97850.0246.732966
TCCAGCA88350.0241.163653
TGGTATC100750.0238.617
GCACGCT90350.0236.313147
AGTGGTA102850.0235.45255
ACGCTTC91050.0234.819129
AGCACGC91050.0234.819126
Produced by FastQC (version 0.11.5)