FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558950
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT16506310.588088136245549TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT739644.744475448218352No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG735414.717341800570897No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA697154.471920202700535No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG340142.1818531704031563No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT212311.3618781872414125No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA209361.3429551942012252No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA173781.1147246544148304No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC168621.0816254530292826No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA134560.8631450655890183No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC101820.6531319157124988No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA92610.5940536899836428No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA87060.558452804772443No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC85330.5473555919048076No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC70540.45248404374739415TruSeq Adapter, Index 27 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC61500.39449629558356586No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC56820.3644760896757433No Hit
GAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGT53680.34433432759228966TruSeq Adapter, Index 27 (97% over 34bp)
AGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCTC46220.2964816062093075No Hit
AGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTGC43910.2816639404727541No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC41590.26678212899708137No Hit
AGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCAC40150.2575451425639052No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG36440.23374707335065267TruSeq Adapter, Index 27 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC29670.1903204079669008No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA28050.1799287982295776No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGACCCCCCAGCCTGCGACTCT24300.15587414605984798No Hit
AGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTGC22470.14413547580101993No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG20310.13027999615125566No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGTCCCTGAGACTCTC16510.10590461528592963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT498300.0285.139651
TGTCCAG531300.0283.196041
AGCAGTG544850.0260.124632
GTCCAGC592100.0253.860022
TCCAGCA599300.0250.810123
GTGGTAT571050.0245.024896
CCAGCAC615050.0244.220214
CAGCACG622550.0241.136435
AGCACGC624650.0240.419866
TGGTATC585400.0238.817757
GCAGTGG596600.0237.043763
CACGCTT640400.0234.965888
GCACGCT639700.0234.832497
ACGCTTC643200.0233.828819
GGTATCA602100.0232.169458
GTATCAA603150.0230.985649
CAGTGGT623400.0224.708224
AGTGGTA631100.0221.524195
GATCGGA422200.0134.467821
ATCGGAA427100.0132.839252
Produced by FastQC (version 0.11.5)