FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558950
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT16783110.765643542127714Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT890975.715192918310401No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG825185.293178100644664No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA783545.026075242951987No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG380552.4410661021841626No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA250981.6099297604156644No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT240121.5402674877321274No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA206611.3253151159434235No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC184761.1851566759677987No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA152990.9813656627858495No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA116010.7441547195227557No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA112490.7215754193527695No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC112380.7208698162224575No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC106810.6851406395330191No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC80070.513614933128067No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC73440.471086308091985No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC47090.3020622855126848No Hit
AAGCAGTGGTATCAACGCAGAGTCTCAGGGACCCCCCAGCCTGCGACTCT41530.2663972545623657No Hit
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC33060.21206581352833637No Hit
AGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCTC30420.19513133840084673No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA30130.19327111196638763No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTCGGTG28570.18326437666378012Illumina Single End PCR Primer 1 (96% over 29bp)
AGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTGC27740.17794028031687995No Hit
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG25240.16190384553706022No Hit
AGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCAC23390.15003688379999358No Hit
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGTCCCTGAGACTCTC17880.11469258154527086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT601350.0286.016751
TGTCCAG463600.0283.09651
AGCAGTG645900.0265.616732
GTGGTAT650650.0257.7846
TGGTATC661250.0252.904337
GCAGTGG685450.0249.626363
GGTATCA675800.0247.30718
GTATCAA676200.0246.554419
GTCCAGC537350.0244.247162
TCCAGCA542750.0241.708773
CAGTGGT715850.0236.028934
CCAGCAC558050.0235.081884
AGCACGC559850.0234.640966
CAGCACG560400.0234.148535
AGTGGTA724050.0232.381855
CACGCTT568850.0231.194348
GCACGCT568650.0230.83647
ACGCTTC571350.0230.230589
GATCGGA418750.0134.669051
ATCGGAA419250.0134.394582
Produced by FastQC (version 0.11.5)