FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378721
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT397142.8804957638274895No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT392912.8498151547702544No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA385642.7970851245465904No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT367302.664063287641227No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG340622.470550604509542No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA312402.265868148813284No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG304162.2061026124937535No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA292852.124070062035756No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG241931.7547422574980727No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT226941.646018302470188No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA225641.6365892736819123No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC131030.9503735708674923No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG128570.9325309471604479No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGTCTGGTCCTACGCTGGTGAA119520.8668904005959146No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC117500.852239140478748No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT115150.8351943576691732Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC113610.8240245851046006No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC112070.812854812540028No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA84520.6130319332192663No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA66720.4839267698105708No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC63700.46202241062550004No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCCGAAGATCTT56690.41117818615949125No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC55320.4012414404364625No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC50700.3677321227427449No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC29120.21121024485737144No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTCAGGGACCCCCCAGTCT29110.21113771386669242No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA28270.2050451106496528No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAG20780.1507193986310501No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAG18680.13548789058845118No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAG18430.1336746158214751No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT609500.0285.935421
TGTCCAG491050.0274.16661
AGCAGTG659550.0263.78432
GTCCAGC516300.0259.822632
GTGGTAT651600.0257.992436
TCCAGCA524450.0255.728933
TGGTATC667950.0251.06147
CCAGCAC538300.0248.630634
AGCACGC541100.0247.353036
AGTGGTA686300.0245.740235
GGTATCA686200.0244.212928
CAGTGGT697850.0244.043414
GTATCAA685800.0243.614329
CACGCTT551550.0242.293538
CAGCACG554750.0241.001865
ACGCTTC554450.0240.9829
GCAGTGG724850.0238.763853
GCACGCT589000.0226.863047
GGGTATC15900.0127.528138
AAGCAGG13750.0118.04431
Produced by FastQC (version 0.11.5)