FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597396
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT21077613.194974821522026TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT819655.131163468545057No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT483813.028741777242462No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA463422.901096534610078No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG352842.208844895066721No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT334932.096724919806986No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA315491.9750268562084794No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA194181.2156033945245888No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC191291.1975114498846873No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG189581.1868065276237076No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA175221.0969102213853046No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG132920.8321042496663319No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC83700.5239777738269032No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC81410.5096419422610298No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC78080.4887955147001745No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC74450.46607103060230526No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC67120.4201838492145967No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT47660.2983605818469559No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA47430.29692073850191186No Hit
AGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCTC42840.2681864734855978No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA40220.251784779729009No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA40060.25078314957593484No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG36630.22931070316940821TruSeq Adapter, Index 27 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC33340.20871468314682143TruSeq Adapter, Index 27 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG28230.17672512013301647No Hit
AGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCTC26990.16896248644669198No Hit
GTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCTCCTGT21030.13165176324468073No Hit
GAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGT20610.12902248409286113TruSeq Adapter, Index 27 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT19340.12107204475283524TruSeq Adapter, Index 27 (97% over 39bp)
AGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCTCCTG18650.11675251471770305No Hit
AGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCTC18070.11312160541280936No Hit
AGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTGC18030.11287119787454082No Hit
AGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCAC16740.10479555476538066No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC16270.1018532661907254No Hit
GCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCTCC16240.10166546053702401No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG559850.0287.008641
AAGCAGT507850.0283.581671
AGCAGTG557150.0257.873962
GTCCAGC623900.0257.418272
TCCAGCA632100.0254.218413
CCAGCAC647900.0248.041584
CAGCACG650550.0246.873065
AGCACGC656350.0244.557176
CACGCTT665600.0241.313058
GCACGCT665750.0241.192467
ACGCTTC666850.0240.838679
GTGGTAT581700.0240.573996
GCAGTGG620850.0229.545873
TGGTATC610300.0229.276117
GGTATCA623250.0224.818658
GTATCAA621650.0224.78279
CAGTGGT631100.0223.815194
AGTGGTA640550.0218.677935
GATCGGA373150.0183.08961
ATCGGAA376850.0181.325292
Produced by FastQC (version 0.11.5)