FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597396
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT18848811.799704018289766Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCT934335.849081880760938No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT547743.428955625280143No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCTTCGGAGACCCTGTCCCTCA527523.3023746146854007No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGGCCTCAGTGAAGGTCTCCTG408982.5602918750266057No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT382182.392518824386689No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA356622.232508407433097No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA220161.3782430906300003No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGCGGGGAGTCTCTGAAGATCTC214191.340869765543422No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG203241.2723207019424112No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGGTCTGGTCCTACGCTGGTGAA174761.0940305346952164No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG144840.9067256960703546No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC90570.5669852685245237No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC90340.5655454251794796No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC89260.5587844216462292No Hit
AAGCAGTGGTATCAACGCAGAGAACTCCGTCGCAGACCCTCTCACTCACC83850.5249168020954103No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC73350.4591848232999206No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTGGGGGGTCCCTGAGACTCT51300.3211476678293923No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA47390.29667033096364337No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTCTTCGGAGACCCTGTCCCTCA45350.2838995465119482No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCGCCTGTGTAGATCT43410.2717547809059244Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA33640.21059273968383543No Hit
AGCAGTGGTATCAACGCAGAGAACTCCGCTGGGGGGTCCCTGAGACTCTC32270.20201628149813822No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTGGCCTCAGTGAAGGTCTCCTG30080.18830646877793608No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCGCCTGTGTAGATCT23930.1498063097691493Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTACGCCTGTGTAGATCT19910.12464035217316183Illumina Single End PCR Primer 1 (96% over 32bp)
AGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCTC18830.11787934863991145No Hit
AAGCAGTGGTATCAACGCAGAGTTGAAGTTCGCAGACCCTCTCACTCACC18660.1168151166022702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG512150.0286.450741
AAGCAGT591400.0286.26171
AGCAGTG634450.0266.189942
GTGGTAT633650.0255.928996
GTCCAGC575000.0254.768172
TCCAGCA581150.0251.920413
TGGTATC654700.0246.847497
CCAGCAC596700.0245.059864
CAGCACG597450.0244.801425
AGCACGC602050.0243.053026
GCAGTGG691650.0242.285113
GTATCAA667350.0241.633049
CACGCTT605650.0241.591618
ACGCTTC606550.0241.119579
GGTATCA671500.0240.613628
GCACGCT608500.0240.5257
CAGTGGT696400.0237.467714
AGTGGTA690150.0235.360265
ATCGGAA368950.0183.909552
GATCGGA368800.0183.833341
Produced by FastQC (version 0.11.5)