FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518789
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT35428123.326545030283995TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTG360122.3710996063310965Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCT324862.138940958882373Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA322372.122546318152159Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC264361.7405972784896386Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTGGGGGGTCCCTGAGACTCT224731.4796657073497372Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACTTCGGAGACCCTGTCCCTCA197151.2980736626351652Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCTTCGGAGACCCTGTCCCTCA170781.1244484915284478Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCACGGGGAGTCTCTGAAGATCTC114500.7538901058672403Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC108950.7173478343601383Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG105400.6939739489817217Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT94830.6243790282916192Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTGGCCTCAGTGAAGGTCTCCTG86620.5703228032333655Clontech Universal Primer Mix Long (95% over 22bp)
GAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGT82040.5401671989986759TruSeq Adapter, Index 27 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTCGGGGAGTCTCTGAAGATCTC79300.5221265100023769Clontech Universal Primer Mix Long (95% over 22bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC75290.4957238958143626TruSeq Adapter, Index 27 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA61820.4070348152376663Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCATCGCAGACCCTCTCACTCACC55050.3624598281920662Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTTCGCAGACCCTCTCACTCACC48400.3186749443141872Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACGGGGAGTCTCTGAAGATCTC48230.3175556314932489Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA38500.25349143297719434Clontech Universal Primer Mix Long (95% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG37050.24394435303389742TruSeq Adapter, Index 27 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT35640.23466064081317417Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGCCTAGGTGTCTGGTCCTACGCTGGTGAA33940.22346751260379155Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGCTCTGCAGTCTGGTCCTACGCTGGTGAA32400.21332785528470377Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT31840.2096407071686719TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGGGATCAATCGCAGACCCTCTCACTCACC29170.19206091168687686Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAAGTCTGGTCCTACGCTGGTGAA27220.17922173521140855Clontech Universal Primer Mix Long (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGACCTCGTAT22140.1457740344445476TruSeq Adapter, Index 27 (97% over 39bp)
AGCAGTGGTATCAACGCAGAGCTCTGCAGGCCTCAGTGAAGGTCTCCTGC16550.10896839521487185Clontech Universal Primer Mix Long (95% over 21bp)
AGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTCC15680.10324014724889369Clontech Universal Primer Mix Long (100% over 22bp)
AGCAGTGGTATCAACGCAGAGCCTAGGTCTGGGGGGTCCCTGAGACTCTC15520.10218667635859886Clontech Universal Primer Mix Long (95% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)