FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926841
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT67402034.98057182715128TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT421262.186272764592408No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA324511.6841555686224239No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG319051.6558190322917148No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT224621.1657422693413728No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA215551.1186704040447553No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC194321.0084900622313933No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT187140.9712269979723288No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA162840.8451138417752165No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA136270.7072197446494028No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG117810.611415264674148No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC112140.5819888615614885No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG88690.4602870709103657No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC80110.41575822810496554No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC78760.40875194164957046TruSeq Adapter, Index 27 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA77090.4000849058121558No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT72430.37590024293649554No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC67010.3477713002785388No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGTCTGGTCCTACGCTGGTGAA65470.3397789438775696No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTAT58240.30225638752756456TruSeq Adapter, Index 27 (97% over 39bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC52430.27210340656027143No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC52220.2710135397783211No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC51520.26738065050515325No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA49200.2553402174855113No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG44020.2284568368640692No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG43050.22342269029982237TruSeq Adapter, Index 27 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA39660.20582912653405236No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGACCTCGTAT39620.20562153286129994TruSeq Adapter, Index 27 (97% over 39bp)
GAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCGT36940.1917127567868859TruSeq Adapter, Index 27 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC30900.16036611220126623No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCATCTCGTATG25590.1328080521433787TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAACTCGTAT24480.12704732772449828TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT23100.11988534601453883TruSeq Adapter, Index 27 (97% over 39bp)
AGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCTC22280.11562967572311363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAG504850.0276.075381
AAGCAGT427650.0273.74761
GTCCAGC529500.0262.987462
TCCAGCA539300.0258.29033
AGCAGTG452650.0257.835882
CCAGCAC553200.0251.747244
CAGCACG562350.0247.59885
AGCACGC568800.0244.739446
GTGGTAT472050.0241.791636
GCACGCT582950.0238.849267
GATCGGA915900.0238.691311
CACGCTT584300.0238.303598
ACGCTTC584450.0238.141839
TCGGAAG920750.0236.879043
ATCGGAA923250.0236.731062
GCAGTGG499500.0232.152053
CAGTGGT494200.0232.143974
TGGTATC492100.0231.522087
CGGAAGA941700.0231.297124
AGTGGTA501250.0227.940755
Produced by FastQC (version 0.11.5)