FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926841
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT59794031.032140171399714Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT487322.529113715143076No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA364961.8940846701933374No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG340621.7677639203234725No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT252861.3123034023046012No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA244111.266892286390003No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC206001.0691074146751083No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT204941.063606182347168No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGCCCTGGTGTAGATCT187950.9754307698455658Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA187760.9744446998999918No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGGCCTGGTGTAGATCT181270.940762626495907Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA162470.8431936003022564No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGACCTGGTGTAGATCT161590.8386265395017025Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG121060.6282822505852844No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC114410.59376980249019No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT103820.5388093776289792No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG97330.5051273042248945No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA85890.4457555138176943No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC81980.4254632323061425No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC73610.38202425628269276No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGTCTGGTCCTACGCTGGTGAA70330.365001575116992No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC69290.35960413962542837No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC65080.3377549055682332No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC56540.2934336564355855No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGTCTGGTCCTACGCTGGTGAA50170.2603743640497581No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC48010.2491643057211259No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG46260.24008208253820632No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA43490.2257062207000993No Hit
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT20880.10836389717677795Illumina Single End PCR Primer 1 (96% over 33bp)
AGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCTC20860.10826010034040172No Hit
GATCGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC20820.10805250666764928Illumina Single End PCR Primer 1 (96% over 28bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTCGGTG19520.10130571230319473Illumina Single End PCR Primer 1 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT497850.0275.656461
TGTCCAG398450.0274.50941
AGCAGTG532500.0256.83582
GTGGTAT539300.0246.492816
GATCGGA906050.0239.990461
TGGTATC555000.0239.36117
TCGGAAG907300.0239.014823
ATCGGAA909500.0238.759772
GTCCAGC460900.0237.247562
CAGTGGT565600.0237.02534
GCAGTGG573100.0236.897453
GAGCGTC907000.0236.892939
CGGAAGA917950.0236.097724
AGAGCGT913800.0235.654658
AAGAGCG920850.0234.562457
GGTATCA569300.0233.483788
AGTGGTA572150.0233.393375
GGAAGAG934700.0232.092915
GAAGAGC933450.0231.758326
TCCAGCA472450.0231.540863
Produced by FastQC (version 0.11.5)