FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460006
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT6431413.981121985365409TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT369258.027069212140711Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA175273.810167693464868Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA115062.501271722542749Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG92442.009539006012965Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC57361.2469402573009916Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT49871.084116294135294Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA39210.8523801863453955Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTC38200.8304239509919437Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT32370.7036864736546915Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC29160.6339047751551067Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC27480.5973835123889689Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC21520.46781998495671795Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT19090.4149945870271257Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCAC18430.40064694808328594Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA15560.3382564575244671Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA14820.32216971082985874Clontech Universal Primer Mix Long (95% over 22bp)
AGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCCTG14380.3126046182006322No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC13290.2889092750964118Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC13200.28695277887679727Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCAC11450.248909796828737Clontech Universal Primer Mix Long (100% over 22bp)
GTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCCTGT10210.22195362669182578No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA9820.21347547640682948Clontech Universal Primer Mix Long (95% over 22bp)
GAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCCGT9540.20738859927913983TruSeq Adapter, Index 6 (96% over 31bp)
AGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTGC9270.20151911062029626Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG9120.1982582835876054Clontech Universal Primer Mix Long (95% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG8150.1771716021095377TruSeq Adapter, Index 6 (97% over 35bp)
GTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCCTGTGCA7330.1593457476641609No Hit
GCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCC7320.15912835919531484No Hit
AGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCTC6320.13738951231070898Clontech Universal Primer Mix Long (95% over 21bp)
AGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCACCTG5740.1247809811176376No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG5160.11217244992456621Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCAC5110.1110855075803359Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTC4820.10478124198380022No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGTCCCTGAGACTCTC4760.10347691117072386Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTC4730.10282474576418568No Hit
AGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACCT4630.10065086107572509Clontech Universal Primer Mix Long (100% over 22bp)
CAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCCT4610.10021608413803297No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)