FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460006
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT6184313.443955078846798Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCT408988.890753598866102Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCA202094.393203566909997Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCA126842.7573553388434062Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTG108782.3647517641074245Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGTCGCAGACCCTCTCACTCACC60821.322156667521728Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTGGGGGGTCCCTGAGACTCT52421.139550353691039Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCTGGGGGGTCCCTGAGACTCT43390.943248566323048Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACTTCGGAGACCCTGTCCCTCA41860.909988130589601Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATCGGGGAGTCTCTGAAGATCTC31520.6852084538027765Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGATCGCAGACCCTCTCACTCACC29570.642817702377795Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGCGGGGAGTCTCTGAAGATCTC22060.4795589622744051Clontech Universal Primer Mix Long (100% over 23bp)
AGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTC20520.4460811380721121Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACTGGGGGGTCCCTGAGACTCT18900.41086420611905056Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTCGCAGGGTCTGGTCCTACGCTGGTGAA17310.3762994395725273Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATGTCTGGTCCTACGCTGGTGAA15690.34108250761946585Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGCAAGATTCGCAGACCCTCTCACTCACC15180.3299956957083168Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGATGGAGACGGGGAGTCTCTGAAGATCTC13570.2949961522241014Clontech Universal Primer Mix Long (95% over 22bp)
AGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCCTG10640.23130133085220625No Hit
AAGCAGTGGTATCAACGCAGAGATGGAGAGGCCTCAGTGAAGGTCTCCTG10570.22977961157028387Clontech Universal Primer Mix Long (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGGGATCAACTTCGGAGACCCTGTCCCTCA10140.22043190740990334Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGTGGTATCAACGCAGAGGCAAGATCTTCGGAGACCCTGTCCCTCAC10070.21891018812798094Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCCTGT7160.1556501436937779No Hit
AAGCAGTGGTATCAACGCAGAGTCTCAGGGACCCCCCAGCCTGCGACTCT6900.14999804350378038Clontech Universal Primer Mix Long (100% over 23bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTCGGTG6860.14912848962839617Illumina Single End PCR Primer 1 (96% over 29bp)
GTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCCTGTGCA6370.1384764546549393No Hit
GCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTCTCC6130.13325913140263387No Hit
AAGCAGTGGTATCAACGCAGAGGGATCAAGGCCTCAGTGAAGGTCTCCTG5980.129998304369943Clontech Universal Primer Mix Long (95% over 22bp)
AGCAGTGGTATCAACGCAGAGGCAAGATGGCCTCAGTGAAGGTCTCCTGC5950.12934613896340483Clontech Universal Primer Mix Long (95% over 21bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCGATAGTGTAGATCT5880.12782441968148242Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC5560.12086798867840855Illumina Single End PCR Primer 1 (96% over 33bp)
AGCAGTGGTATCAACGCAGAGTCGCAGGCTTCGGAGACCCTGTCCCTCAC5220.11347678073764256Clontech Universal Primer Mix Long (100% over 22bp)
AAGCAGTGGGTATCAACGCAGAGGCAAGATCTGGGGGGTCCCTGAGACTC5110.1110855075803359No Hit
AAGCAGTGGTATCAACGCAGAGGCAAGATCTGGGGGTCCCTGAGACTCTC5100.11086811911148985Clontech Universal Primer Mix Long (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)