FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298712
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT10166434.034119821098585TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT89432.9938536115053966No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT78932.6423444655721897No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA61382.0548220359409735No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT54291.8174696697822652No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT49661.6624708749564798No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA48521.624307024826589No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA34901.1683494469589437No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC24110.8071319531856772No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA23440.7847023219689868No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG22620.7572511315246793No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG19200.6427595811350063No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA12380.4144460215860093No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC11260.37695171268646727No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC9050.3029674067329066No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA8470.2835507110527867No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG8250.276185757518948No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC8020.26848603336993493No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAGCTCGTAT7930.2654730978333646TruSeq Adapter, Index 6 (97% over 36bp)
AGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCTC6750.22597016524277566No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA6520.21827044109376256No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG6470.21659658801789014No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC6410.21458796432684324No Hit
GAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATGCCGT6330.2119097994054474TruSeq Adapter, Index 6 (96% over 31bp)
AGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCTC6230.20856209325370256No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC6180.20688824017783014No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG5620.18814108572805913TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAACCTCGTAT5270.17642411419695225TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC4960.1660462251265433No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC4550.15232062990438952No Hit
AGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCAC4190.14026888775810814No Hit
AGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCTC4030.13491255791531642No Hit
AGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCTC3870.1295562280725247No Hit
AGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCAC3710.12419989822973299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT70900.0281.096621
TGTCCAG83400.0278.617221
GTCCAGC87300.0266.125852
GATCGGA122900.0264.769441
TCGGAAG123550.0263.213443
ATCGGAA124050.0262.507932
TCCAGCA88600.0262.221073
CGGAAGA123950.0261.889834
GGAAGAG126500.0256.494485
CCAGCAC91250.0254.76694
GAAGAGC127800.0253.195476
AGAGCAC127750.0252.834448
CAGCACG92350.0251.891485
AGCACGC92450.0251.301126
CACGCTT92600.0250.894048
GCACGCT92850.0250.218487
ACGCTTC92800.0250.194969
AAGAGCA130300.0248.33757
GAGCACA130850.0246.619869
AGCAGTG80900.0245.764252
Produced by FastQC (version 0.11.5)