FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298712
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT7500625.109804761777234Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCT101353.3929001847933793No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT91573.0654945231527355Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCT81042.7129810653740054No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCA68852.304895685476312No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCTGGGGGGTCCCTGAGACTCT52061.7428158225983557No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCTTCGGAGACCCTGTCCCTCA47391.5864779453118723No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTGGGGGGTCCCTGAGACTCT46281.5493184070275048No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACTTCGGAGACCCTGTCCCTCA32581.0906826642384637No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCGCTATGTGTAGATCT29720.9949382682985619Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCCGGTACGGCCTCAGTGAAGGTCTCCTG28060.9393663461795978No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACCGGGGAGTCTCTGAAGATCTC27890.9336752457216315No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCACTATGTGTAGATCT26020.8710731406840033Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGCTCGATGCTTCGGAGACCCTGTCCCTCA22370.7488818661453173No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGGCCTCAGTGAAGGTCTCCTG18890.6323816920645973No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAAGTCTGGTCCTACGCTGGTGAA11270.3772864833016417No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTCGGGGAGTCTCTGAAGATCTC10760.3602131819277431No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACGTCTGGTCCTACGCTGGTGAA10180.34079648624762315No Hit
AAGCAGTGGTATCAACGCAGAGCCGGTACTCGCAGACCCTCTCACTCACC8450.28288116982243766No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGGGCCTCAGTGAAGGTCTCCTG8000.26781649213958597No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTTCGCAGACCCTCTCACTCACC7570.2534213556870832No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCCATGTGTAGATCT7170.24003053108010391Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCAGTGGTATCAACGCAGAGGCTCGAAGGCCTCAGTGAAGGTCTCCTG6400.21425319371166876No Hit
AAGCAGTGGTATCAACGCAGAGACCAACTGTCTGGTCCTACGCTGGTGAA6200.2075577814081791No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAACGGGGAGTCTCTGAAGATCTC5850.1958408098770722No Hit
AAGCAGTGGTATCAACGCAGAGGCTCGAATCGCAGACCCTCTCACTCACC5400.18077613219422053No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGCGGGGAGTCTCTGAAGATCTC5270.17642411419695225No Hit
AGCAGTGGTATCAACGCAGAGCCGGTACCTGGGGGGTCCCTGAGACTCTC5110.17106778435416053No Hit
AAGCAGTGGTATCAACGCAGAGCTCGATGTCGCAGACCCTCTCACTCACC4190.14026888775810814No Hit
AGCAGTGGTATCAACGCAGAGACCAACTCTGGGGGGTCCCTGAGACTCTC3710.12419989822973299No Hit
AGCAGTGGTATCAACGCAGAGCCGGTACCTTCGGAGACCCTGTCCCTCAC3480.11650017408071989No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTCGGTG3310.1108090736227537Illumina Single End PCR Primer 1 (96% over 27bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCCATGTGTAGATCT3270.10946999116205576Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT80150.0277.960141
TGTCCAG70850.0276.987271
GATCGGA119850.0267.204251
TCGGAAG120050.0265.57663
ATCGGAA120500.0265.072542
GAGCGTC119450.0264.125829
CGGAAGA120750.0263.550324
AGAGCGT121900.0260.143348
AAGAGCG122100.0260.034767
GGAAGAG122500.0259.905275
GAAGAGC123150.0258.294836
GTGGTAT86000.0253.529056
AGCAGTG88300.0252.416142
TGGTATC88000.0246.93227
GGTATCA89400.0242.94168
GTATCAA89450.0241.327279
GTCCAGC82350.0238.539372
CAGTGGT92350.0238.164584
GCAGTGG93850.0236.080053
TCCAGCA84050.0234.93833
Produced by FastQC (version 0.11.5)