FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469261
Sequences flagged as poor quality0
Sequence length300
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT19702813.410006799336537TruSeq Adapter, Index 7 (97% over 38bp)
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA421392.8680404638794603No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA234221.5941347384841769No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC229741.563643219278263No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC180371.2276239551720218No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA154451.0512087369092353No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA141700.9644304177406193No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA141320.9618440835222606No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA125340.853081923497595No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA84840.5774331449619912No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA79840.5434024315625339No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC58430.3976829167860577No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC58410.3975467939324599TruSeq Adapter, Index 7 (97% over 35bp)
GAAGCCCAAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGC57320.39012809841137824No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA55280.3762435673443997No Hit
AGTGCTGTCATCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACACGT53260.36249515913101893No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAACTCGTAT51920.3533749279399644TruSeq Adapter, Index 7 (97% over 38bp)
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC45200.3076376491310938No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCACCTCGTAT44750.3045748849251427TruSeq Adapter, Index 7 (97% over 38bp)
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC30050.20452458753073824No Hit
GACACAGCCGTTTCCCAGAGATGACAGCACTAGATCGGAAGAGCACACGT29500.20078120905679792No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA27250.18546738802704216No Hit
GGTGCTGTCGTCTCTCTGGGTAACATCAGCATCAGATCGGAAGAGCACAC25090.17076611983847662No Hit
GATGCTGATGTTACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC25060.17056193555807989No Hit
GGTGAAGAAGTCGCCCAGGCGGAACGATACGGCAGCGCCGCGGAGCCTCG24340.165661512828558No Hit
GAAGCTGGAGTGGCCCAGTCTCCCAGATATAAGATTATAGAGAAAAGGCA24250.1650489599873678No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACCAGATCGGAAGAGCACACGT23510.16001241440424813No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA23370.1590595544290633No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC23290.158515063014672No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT22810.1552481145283241TruSeq Adapter, Index 7 (97% over 38bp)
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACACGT22750.1548397459675306No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC20270.13796051212139981No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCCGT19100.1299973251859268TruSeq Adapter, Index 7 (96% over 33bp)
GATGTGAAAGTAACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC18870.12843191236955176No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC18860.12836385094275285No Hit
GGTGCTGTCGTCTCTCTGGGTTACTTTCACATCAGATCGGAAGAGCACAC18290.12448434961521472No Hit
ACTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG16460.11202910851101336No Hit
GATGCCATGGTCATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT16080.1094427742926546No Hit
GAAGCCCAAGTGACCCAGGCTGGTGACCTTCCTGGACAGCAGCAACAGGC15840.10780930004948065No Hit
GGTGAAGAAGTCGCCCAGATGCACCAGAACTTCTCGCAGCAGCTGCTCCA15720.10699256292789369No Hit
GAAGCCCAAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGC15640.10644807151350237No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG15560.10590358009911105TruSeq Adapter, Index 7 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCTT247100.0272.741581
CCGGCGT39050.0265.254945
AATGCCG39050.0265.254941
GAAGTCG67150.0258.841647
GGCGTCA40100.0258.675757
CGGCGTC40150.0257.987676
GCGTCAT40500.0256.48378
GCTCTTC269650.0249.71512
ATGCCGG41800.0247.803962
TGTCGTC66100.0247.171666
GAAAGTA62950.0246.703116
AGTAACC63000.0246.274129
GAAGCCC88250.0245.402041
AGCCGTT40050.0242.857136
CTGTCGT67250.0242.726495
GTCGTCT67650.0241.725657
CGTCATG43100.0240.670479
GAACCTG91700.0238.412461
GTGCTGT117800.0238.34762
AAGTAAC65150.0238.146878
Produced by FastQC (version 0.11.5)