FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469261
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT20362513.859008031928976Illumina Single End PCR Primer 1 (97% over 34bp)
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA571113.8870561459128092No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC373412.5414817380982684No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA263051.7903558319454473No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC207741.413908080320651No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA180101.2257862966484512No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA169081.1507826043160474No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA166821.1354007218594926No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA161161.096877954291307No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA104430.7107654800610647No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA103560.7048441359295592No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA93380.6355576034482642No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC78240.5325126032747074No Hit
GAAGCCCAAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGC68920.4690793534981191No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC64670.44015324710858045No Hit
GACACAGCCGTTTCCCAGAGATGACAGCACTAGATCGGAAGAGCGTCGTG54810.3730446802848507No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC44130.3003550764636099No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC41440.28204655265470197No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA37200.25318850769196216No Hit
AGTGCTGTCATCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCGTG30700.20894858027266772No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA30000.2041842803967437No Hit
GAAGCTGGAGTGGCCCAGTCTCCCAGATATAAGATTATAGAGAAAAGGCA28670.19513211063248803No Hit
GGTGCTGTCGTCTCTCTGGGTAACATCAGCATCAGATCGGAAGAGCGTCG25960.1766874639699822No Hit
GATGCTGATGTTACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG25870.17607491112879198No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCGTG24440.1663421270965472No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACCAGATCGGAAGAGCGTCGTG23210.1579705716002807No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG20940.14252062771692708No Hit
AGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC20290.13809663497499763No Hit
GGTGCTGTCGTCTCTCTGGGTTACTTTCACATCAGATCGGAAGAGCGTCG19720.1342171336474595No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG19460.13244753655068772No Hit
GATGTGAAAGTAACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG18880.1284999737963507No Hit
GGTGAAGAAGTCGCCCAGGCGGAACGATACGGCAGCGCCGCGGAGCCTCG18790.12788742095516045No Hit
AATGCCGGCGTCATGCAGCTGCATCCACAGTTGCAGAGCCTTGAAACCAA18580.12645813099238326No Hit
GGTGAAGAAGTCGCCCAGATGCACCAGAACTTCTCGCAGCAGCTGCTCCA17690.12040066400727985No Hit
ACTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG17570.11958392688569286No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA17380.11829075977651349No Hit
GAAGCCCAAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGC17090.11631697839934498No Hit
AGCTGGAGTCTCCCAGTCCCCCAGTAACAAGGTCACAGAGAAGGGAAAGG16450.11196104708421444No Hit
GACACAGCCGTTTCCCTTCATTCTTTAAAAGATGACAACTGGAGAGCTTT16120.10971501999985027No Hit
GATGCTGATGTTACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG15010.10216020162517075No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATAT14990.10202407877157292Illumina Single End PCR Primer 1 (97% over 34bp)
ACTGGAGTTACGCAGACACCAAGACACCTGGTCATGGGAATGACAAATAA14860.10113928022318704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)