FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173638
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT15254612.997704573301135Illumina Single End PCR Primer 1 (97% over 34bp)
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA12077910.290992622938248No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC709036.041300639549845No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA567814.838033533338218No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC246822.1030334736946146No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA217351.8519339012540492No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA167811.4298275959026547No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC135471.1542741458609895No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA133471.1372331161738116No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC101920.8684108728585817No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA92110.7848246222429744No Hit
GATGGTGGAATCACTCAGTCCCCGAAGTACCTGTTCAGAAAGGAAGGACA60500.515491148037129No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC60160.5125941729903087No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC47220.40233871091426826No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG42270.3601621624385032No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA38670.3294883090015831No Hit
AGTGCTGTCATCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC36910.31449220287686663No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA34720.2958322753694069No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC33450.285011221518049No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA32390.27597947578384474No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC25460.21693230791777363No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATAT21450.18276504339498212Illumina Single End PCR Primer 1 (97% over 34bp)
AGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGA20150.17168837409831653No Hit
GATGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA19340.1647867570750095No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA19300.16444593648126596No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA18810.1602708842079074No Hit
GCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAA18100.15422131866895925No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCGGTGCTGTCGTCTCTCT17280.14723449649721634No Hit
GATGTGAAAGTAACCCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC17090.14561559867693447No Hit
GATGCTGGAATCACCCAGAGCCCAAGATACAAGATCACAGAGACAGGAAG16610.14152575155201177No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA16210.1381175456145762No Hit
GGTGCTGTCATCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC16190.13794713531770444No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGAACT16030.13658385294273023Illumina Single End PCR Primer 1 (97% over 34bp)
GTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAACC15280.13019346681003854No Hit
GTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAACCTCTGT14830.12635923513042352No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA14250.12141733652114195No Hit
GGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC13970.11903159236493707No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA12870.10965902603698927No Hit
ATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACAG12770.10880697455263037No Hit
GATGTGAAAGTAACCCAGAAGCGTGCTGCAAGAATAAGATCGGAAGAGCG12430.10590999950581015No Hit
GACACAGCCGTTTCCCAGAGACGACAGCACCGACACAGCCGTTTCCCAGA12060.10275740901368223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)