FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148822
Sequences flagged as poor quality0
Sequence length300
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT934448.13389715726196TruSeq Adapter, Index 13 (97% over 38bp)
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC522484.547963043883212No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA491394.277338003624583No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA403113.5088986805614795No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA330822.8796454106902547No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA277852.4185644077150332No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC265622.312107532759644No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA129001.122889359709337No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC94010.8183165015990294No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC45210.393533550019063No Hit
TGAGTTAACAGCACGCTCTTCCGATCTTGAGTTAACAGCACGCTCTTCCG42810.3726425851872614No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA39950.3477475187626978No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA39100.34034863538476806No Hit
GATGCCATGGTCATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT36690.31937062486616724No Hit
GATGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA34960.3043117210499103No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA33670.2930828274528169No Hit
AATGCCGGCGTCATGAGAGACGACAGCACCAATGCCGGCGTCATGAGAGA33160.28864349742605905No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA31150.2711473143794252No Hit
GATGTGAAAGTAACCCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC29970.26087592333712273No Hit
GGTGCTGTCGTCTCTCATGACGCCGGCATTGGTGCTGTCGTCTCTCATGA29640.25800341567275No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA26230.22832083647423185No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA24980.21744012562433518No Hit
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCAGCACGCTCTTCCG23940.20838737419722114No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC22060.19202278507897655No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG19060.16590907903922453No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAAACTCGTAT19020.16556089629202783TruSeq Adapter, Index 13 (97% over 38bp)
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC18800.16364589118244602No Hit
CGCTCTTCCGATCTTTCTCCATCAGCACTGGTAGATGTCAGCTTCTGATG18000.15668223623851216No Hit
TTCTCCATCAGCACTGGTAGATGTCAGCTTCTGATGGCTCAAACACAGCG16470.14336424615823862No Hit
GTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAACC16180.1408399212410626No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC14590.12699965703999402No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAACCTCGTAT14320.12464942349641632TruSeq Adapter, Index 13 (97% over 38bp)
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAATGCCGGCGTCATGAG14230.12386601231522378No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCTGGTGCTGTCGTCTCTC13960.12151577877164607No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA13230.11516144363530642No Hit
GAAGCTGACATCTACCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC12300.1070661947629833No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC11780.10253981904942627No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)