FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148822
Sequences flagged as poor quality0
Sequence length300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC929598.091679999164361No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA633805.516955629331611No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC552644.810492835269519No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA544614.740595148769784No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA458953.994961795648064No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT425223.7013566940744522Illumina Single End PCR Primer 1 (96% over 32bp)
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA329042.8641512784400023No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA122221.0638723840594975No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC107230.9333908995475365No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATAT72410.6302978181128147Illumina Single End PCR Primer 1 (96% over 32bp)
GATGTGAAAGTAACCCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC60110.5232316233498314No Hit
GATGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA51220.44584800778536626No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC50730.44158276913220673No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA47960.4174711138888357No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA41690.36289346826575397No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAACT36050.31379970091102016Illumina Single End PCR Primer 1 (96% over 32bp)
GGTGCTGTCGTCTCTCATGACGCCGGCATTGGTGCTGTCGTCTCTCATGA34770.30265785300072595No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC34150.29726102041917724No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA33370.2904714568488417No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA30350.2641836594354913No Hit
AATGCCGGCGTCATGAGAGACGACAGCACCAATGCCGGCGTCATGAGAGA28090.24451133421887813No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATTT28050.24416315147168144Illumina Single End PCR Primer 1 (96% over 32bp)
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG27230.23702540515414922No Hit
GATGCCATGGTCATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT23000.20020507963809886No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA22360.19463415568295175No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC20900.18192548541027243No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA20280.17652865282872368No Hit
GGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC19480.16956499788478982No Hit
GTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAACC18980.16521271354483114No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC18920.1646904394240361No Hit
GAAGCTGACATCTACCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC18410.16025110939727824No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC17210.14980562698137745No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAATGCCGGCGTCATGAG16300.14188446948265265No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC16260.14153628673545599No Hit
CGCTCTTCCGATCTTTCTCCATCAGCACTGGTAGATGTCAGCTTCTGATG15490.1348337688519196No Hit
CTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGAA14620.1272607941003915No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCA13690.11916554522806841Illumina Single End PCR Primer 1 (96% over 32bp)
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGACACAGCCGTTTCCC13440.11698940305808908No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGAAAT13130.1142909867673147Illumina Single End PCR Primer 1 (96% over 32bp)
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCTGGTGCTGTCGTCTCTC12920.11246302734453206No Hit
GTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAACC12760.11107029635574527No Hit
ATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACAG11800.10271391042302462No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG11530.10036367687944694No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAATGCCGGCGTCATGAG11500.10010253981904943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCTG65750.0253.360951
GGAATCA38900.0252.409067
CGCTCTT241600.0250.281191
TGGAATC39750.0246.27216
TGAAGTC68500.0244.834816
AACCTGA69350.0240.44712
GAATCAC40650.0240.169598
GAGTCAT93250.0236.354178
GCTCTTC257800.0234.051742
GAAGTCA71700.0233.292727
CTGAAGT74750.0232.622455
CCATGGT17200.0232.44415
AGTCATC96100.0229.966749
GAAGCCC107300.0227.939871
AAGTCAC74850.0225.11448
TCTTCCG269550.0223.08614
CTCTTCC269750.0222.975173
GATGGTG20600.0222.168351
GTGGAAT20700.0220.125035
GAAGTCG19900.0218.633517
Produced by FastQC (version 0.11.5)