FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6465
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCAGCACGCTCTTCCG1171.8097447795823667No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC1021.5777262180974478No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC961.4849187935034802No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA841.2993039443155452No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA600.9280742459396751No Hit
GAACCTGAAGTCACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC580.897138437741686No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC570.8816705336426913No Hit
GAACCTGAAGTCACCCAGCTGGGTCACTTGGGCTTCAGATCGGAAGAGCA540.8352668213457077No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC420.6496519721577726No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA420.6496519721577726No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA360.5568445475638051No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC360.5568445475638051No Hit
GAAGCCCAAGTGACCCCAGCAGGGTGACTTCAGGTTCAGATCGGAAGAGC350.5413766434648105No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC330.5104408352668214No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC330.5104408352668214No Hit
GATGCTGATGTTACCCAGGGCGACTTCTTCACCAGATCGGAAGAGCACAC300.46403712296983757No Hit
TTCTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA290.448569218870843RNA PCR Primer, Index 14 (100% over 28bp)
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC280.4331013147718484No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA260.40216550657385924No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC250.3866976024748647No Hit
GGTGAAGAAGTCGCCCTGGGTAACATCAGCATCAGATCGGAAGAGCACAC240.37122969837587005No Hit
GAAGCCCAAGTGACCCAGCTGGGTGACTTCAGGTTCAGATCGGAAGAGCA240.37122969837587005No Hit
GCTGCTGGAGTCATCCCTGGGTAACATCAGCATCAGATCGGAAGAGCACA240.37122969837587005No Hit
GCTGCTGGAGTCATCCTGGGTTACTTTCACATCAGATCGGAAGAGCACAC230.3557617942768755No Hit
GATGCTGATGTTACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC220.3402938901778809No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA210.3248259860788863No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC200.30935808197989173No Hit
GCTGCTGGAGTCATCCAGGGTAACATCAGCATCAGATCGGAAGAGCACAC180.27842227378190254No Hit
GAAGCCCAAGTGACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCACAC170.262954369682908No Hit
GATGTGAAAGTAACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC170.262954369682908No Hit
GATGCTGATGTTACCCTGGATGACTCCAGCAGCAGATCGGAAGAGCACAC170.262954369682908No Hit
GCTGCTGGAGTCATCCTGGGTAACATCAGCATCAGATCGGAAGAGCACAC160.24748646558391338No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT160.24748646558391338TruSeq Adapter, Index 13 (97% over 38bp)
GAACCTGAAGTCACCCTGCTGGGTCACTTGGGCTTCAGATCGGAAGAGCA150.23201856148491878No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC150.23201856148491878No Hit
GATGTGAAAGTAACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCACAC150.23201856148491878No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC150.23201856148491878No Hit
GATGCTGATGTTACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCACAC140.2165506573859242No Hit
GGTGCTGTCGTCTCTCTGGGTTACTTTCACATCAGATCGGAAGAGCACAC130.20108275328692962No Hit
GAAGCCCAAGTGACCCCAGCATGACGCCGGCATTAGATCGGAAGAGCACA130.20108275328692962No Hit
GATGCCATGGTCATCCAGGGCGACTTCTTCACCAGATCGGAAGAGCACAC130.20108275328692962No Hit
AATGCCGGCGTCATGAGAGACGACAGCACCAGATCGGAAGAGCACACGTC130.20108275328692962No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC130.20108275328692962No Hit
AGTGCTGTCATCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC130.20108275328692962No Hit
GAACCTGAAGTCACCCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC130.20108275328692962No Hit
GCTGCTGGAGTCATCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC120.18561484918793503No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA120.18561484918793503No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAGAGATTCCATCTCGTATG110.17014694508894046TruSeq Adapter, Index 13 (97% over 35bp)
GGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC110.17014694508894046No Hit
GGTGAAGAAGTCGCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC110.17014694508894046No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA110.17014694508894046No Hit
TTTGATCCCAGCACGCTCTTCCGATCTTTTGATCCCAGCACGCTCTTCCG110.17014694508894046No Hit
TTTAGCTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAA100.15467904098994587RNA PCR Primer, Index 14 (100% over 28bp)
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCAGCACGCTTCAGGC100.15467904098994587No Hit
GCTGCTGGAGTCATCCAGGGCGACTTCTTCACCAGATCGGAAGAGCACAC100.15467904098994587No Hit
GAACCTGAAGTCACCCATGACGCCGGCATTAGATCGGAAGAGCACACGTC100.15467904098994587No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGCACGAGATTCCATCTCGTATG100.15467904098994587Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GGTGAAGAAGTCGCCCATGACGCCGGCATTAGATCGGAAGAGCACACGTC100.15467904098994587No Hit
GGTGCTGTCGTCTCTCATGACGCCGGCATTAGATCGGAAGAGCACACGTC100.15467904098994587No Hit
ACTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG100.15467904098994587No Hit
GATGCTGATGTTACCCAGGGATGACTCCAGCAGCAGATCGGAAGAGCACA90.13921113689095127No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT90.13921113689095127TruSeq Adapter, Index 7 (97% over 38bp)
AGTGCTGTCATCTCTCTGGGCGACTTCTTCACCAGATCGGAAGAGCACAC90.13921113689095127No Hit
TACTCCAGTCACGAGATTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAA90.13921113689095127RNA PCR Primer, Index 14 (100% over 28bp)
TTTGATCCCAGCACCGCTCTTCCGATCTTTTGATCCCAGCACCGCTCTTC90.13921113689095127No Hit
AATGCCGGCGTCATGGGCGACTTCTTCACCAGATCGGAAGAGCACACGTC90.13921113689095127No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC90.13921113689095127No Hit
GACACAGCCGTTTCCCAGTGTGACTCCAGAATCAGATCGGAAGAGCACAC90.13921113689095127No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC90.13921113689095127No Hit
AATGCCGGCGTCATGCAGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC90.13921113689095127No Hit
GGTGCTGTCGTCTCTCTGGGTAACATCAGCATCAGATCGGAAGAGCACAC90.13921113689095127No Hit
GAAGCCCAAGTGACCCCAGCATGGGTGACTTCAGGTTCAGATCGGAAGAG90.13921113689095127No Hit
AATGCCGGCGTCATGGGTGACTTCAGGTTCAGATCGGAAGAGCACACGTC90.13921113689095127No Hit
GGTGCTGTCGTCTCTCTGGGCGACTTCTTCACCAGATCGGAAGAGCACAC90.13921113689095127No Hit
GAACCTGAAGTCACCCATGCTGGGGTCACTTGGGCTTCAGATCGGAAGAG80.12374323279195669No Hit
GGTGAAGAAGTCGCCCTGGATGACCATGGCATCAGATCGGAAGAGCACAC80.12374323279195669No Hit
AATGCCGGCGTCATGCAGGGGTCACTTGGGCTTCAGATCGGAAGAGCACA80.12374323279195669No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACTAGATCGGAAGAGCACAC80.12374323279195669No Hit
TAGGTATTCAGCACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGC80.12374323279195669TruSeq Adapter, Index 9 (95% over 23bp)
AGTGCTGTCATCTCTCCCTGGGTGACTTCAGGTTCAGATCGGAAGAGCAC80.12374323279195669No Hit
AATGCCGGCGTCATGCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC80.12374323279195669No Hit
GCTGCTGGAGTCATCCAGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC80.12374323279195669No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACCAGATCGGAAGAGCACACGT80.12374323279195669No Hit
GATGTGAAAGTAACCCAGCCTGAAGCGTGCTGAATACCTAAGATCGGAAG70.1082753286929621No Hit
CGCTCTTCCGATCTTATCTTGCCAGCACGCTTCAGGCTGGGTTACTTTCA70.1082753286929621No Hit
TATAAGCTCAGCACGGTAGATGTCAGCTTCTGATGGCTCAAACACAGCGA70.1082753286929621No Hit
AATGCCGGCGTCATGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGCACA70.1082753286929621No Hit
GCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG70.1082753286929621No Hit
GGTGAAGAAGTCGCCCAGGGTAACATCAGCATCAGATCGGAAGAGCACAC70.1082753286929621No Hit
GATGGTGGAATCACTCAGTCCCCGAAGTACCTGTTCAGAAAGGAAGGACA70.1082753286929621No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACTAGATCGGAAGAGCACAC70.1082753286929621No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA70.1082753286929621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)