FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005409277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6465
Sequences flagged as poor quality0
Sequence length300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG911.4075792730085075No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG791.2219644238205722No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG570.8816705336426913No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA520.8043310131477185No Hit
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCAGCACGCTCTTCCG470.7269914926527455No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT470.7269914926527455Illumina Single End PCR Primer 1 (96% over 32bp)
GAAGCCCAAGTGACCCAGCTGGGTGACTTCAGGTTCAGATCGGAAGAGCG460.711523588553751No Hit
GAAGCCCAAGTGACCCCAGCAGGGTGACTTCAGGTTCAGATCGGAAGAGC430.6651198762567672No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG390.6032482598607889No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA350.5413766434648105No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG340.525908739365816No Hit
GAACCTGAAGTCACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC310.4795050270688322No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC300.46403712296983757No Hit
GGTGAAGAAGTCGCCCTGGGTAACATCAGCATCAGATCGGAAGAGCGTCG280.4331013147718484No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC250.3866976024748647No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA250.3866976024748647No Hit
GATGCTGATGTTACCCTGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG240.37122969837587005No Hit
GATGCTGATGTTACCCAGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG240.37122969837587005No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG240.37122969837587005No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG240.37122969837587005No Hit
GGTGCTGTCGTCTCTCTGGGTAACATCAGCATCAGATCGGAAGAGCGTCG240.37122969837587005No Hit
GATGTGAAAGTAACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG230.3557617942768755No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA230.3557617942768755No Hit
GATGCTGATGTTACCCAGGGATGACTCCAGCAGCAGATCGGAAGAGCGTC230.3557617942768755No Hit
GATGCTGATGTTACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG190.29389017788089716No Hit
GCTGCTGGAGTCATCCTGGGTTACTTTCACATCAGATCGGAAGAGCGTCG190.29389017788089716No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG190.29389017788089716No Hit
GAACCTGAAGTCACCCAGCTGGGTCACTTGGGCTTCAGATCGGAAGAGCG190.29389017788089716No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA190.29389017788089716No Hit
GCTGCTGGAGTCATCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG180.27842227378190254No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC180.27842227378190254No Hit
GGTGCTGTCGTCTCTCTGGGTTACTTTCACATCAGATCGGAAGAGCGTCG180.27842227378190254No Hit
GATGTGAAAGTAACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG150.23201856148491878No Hit
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCAGCACGCTTCAGGC140.2165506573859242No Hit
GGTGCTGTCGTCTCTCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT140.2165506573859242No Hit
GACACAGCCGTTTCCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG140.2165506573859242No Hit
GAACCTGAAGTCACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG130.20108275328692962No Hit
GGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG130.20108275328692962No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA130.20108275328692962No Hit
GAAGCCCAAGTGACCCAGCAGGGTGACTTCAGGTTCAGATCGGAAGAGCG130.20108275328692962No Hit
GGTGAAGAAGTCGCCCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT130.20108275328692962No Hit
GAAGCCCAAGTGACCCAGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG120.18561484918793503No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG120.18561484918793503No Hit
AGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG110.17014694508894046No Hit
GGTGAAGAAGTCGCCCTGGATGACCATGGCATCAGATCGGAAGAGCGTCG110.17014694508894046No Hit
GGTGAAGAAGTCGCCCTGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG110.17014694508894046No Hit
GAAGCCCAAGTGACCCTGCATGACGCCGGCATTAGATCGGAAGAGCGTCG100.15467904098994587No Hit
AATGCCGGCGTCATGGGCGACTTCTTCACCAGATCGGAAGAGCGTCGTGT100.15467904098994587No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC100.15467904098994587No Hit
AATGCCGGCGTCATGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCGTGT100.15467904098994587No Hit
GATTCTGGAGTCACACTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG100.15467904098994587No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCGTCG90.13921113689095127No Hit
GAACCTGAAGTCACCCATGCTGGGGTCACTTGGGCTTCAGATCGGAAGAG90.13921113689095127No Hit
GCTGCTGGAGTCATCCAGGGTAACATCAGCATCAGATCGGAAGAGCGTCG90.13921113689095127No Hit
GATGTGAAAGTAACCCAGCCTGAAGCGTGCTGGCAAGATAAGATCGGAAG90.13921113689095127No Hit
GATGCTGATGTTACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG90.13921113689095127No Hit
GGTGAAGAAGTCGCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG80.12374323279195669No Hit
GCTGCTGGAGTCATCCTGGGTAACATCAGCATCAGATCGGAAGAGCGTCG80.12374323279195669No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG80.12374323279195669No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG80.12374323279195669No Hit
GAACCTGAAGTCACCCATGACGCCGGCATTAGATCGGAAGAGCGTCGTGT80.12374323279195669No Hit
AGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG80.12374323279195669No Hit
GAAGCCCAAGTGACCCCTGCATGACGCCGGCATTAGATCGGAAGAGCGTC80.12374323279195669No Hit
GAAGCCCAAGTGACCCTGGATGACTCCAGCAGCAGATCGGAAGAGCGTCG80.12374323279195669No Hit
GAACCTGAAGTCACCCAGCATGACGCCGGCATTAGATCGGAAGAGCGTCG70.1082753286929621No Hit
GAACCTGAAGTCACCCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG70.1082753286929621No Hit
AATGCCGGCGTCATGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGCGTC70.1082753286929621No Hit
TACCTAGGCAGCACGCTCTTCCGATCTTACCTAGGCATGACGCCGGCATT70.1082753286929621No Hit
GATGCCATGGTCATCCAGGGCGACTTCTTCACCAGATCGGAAGAGCGTCG70.1082753286929621No Hit
GAACCTGAAGTCACCCAGGGAGAGATGACAGCACTAGATCGGAAGAGCGT70.1082753286929621No Hit
AATGCCGGCGTCATGCAGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG70.1082753286929621No Hit
AGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG70.1082753286929621No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCGTG70.1082753286929621No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGCGCCTGTGTAGATCT70.1082753286929621Illumina Single End PCR Primer 1 (96% over 32bp)
AATGCCGGCGTCATGAGAGACGACAGCACCAGATCGGAAGAGCGTCGTGT70.1082753286929621No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTC108.3557796E-4294.06
GACACAG303.6379788E-12294.01
CCGGCGT108.3557796E-4294.05
AGTGGTA108.3557796E-4294.05
GCCATGG157.0062342E-6294.04
CCATGGT157.0062342E-6294.05
AATGCCG108.3557796E-4294.01
CAGTGGT108.3557796E-4294.04
GTATCAA108.3557796E-4294.09
ATGGTCA108.3557796E-4294.07
GGTGAAG157.0062342E-6294.01
ATGCCGG108.3557796E-4294.02
GTGGTAT108.3557796E-4294.06
GAAGCCC450.0261.333341
TGAAGTC251.7716229E-7235.26
GTGCTGT650.0226.153842
CATGGTC202.2084867E-5220.56
AGCCGTT402.910383E-11220.56
CTGTCGT600.0220.55
TGCCATG202.2084867E-5220.53
Produced by FastQC (version 0.11.5)