FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017332
Sequences flagged as poor quality0
Sequence length300
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT22529111.167770104276341TruSeq Adapter, Index 13 (97% over 38bp)
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA783913.8858750071877113No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA744863.692302506478854No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC721583.5769025623942916No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA376151.8645914504900531No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA204131.0118810389167474No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA185140.9177468061776644No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC171110.8481995031060827No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA163650.8112199677593971No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA120960.5996038331816478No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCGGTGCTGTCGTCTCTCT119500.5923665514650043No Hit
GATGCCATGGTCATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT104880.5198945934531352No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA102770.5094352342599037No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC81020.40161956485100125No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCGGTGCTGTCGTCTCTCT74730.3704397689621738No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC68620.34015224068224764No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA55610.27566112072777316No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC54340.26936567704274755No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC53490.2651521911118249No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC49110.24344034596189423No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAAACTCGTAT48830.24205237412582561TruSeq Adapter, Index 13 (97% over 38bp)
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC46720.23159301493259413No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC46240.22921363464219077No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA44020.2182090007990752No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC41780.20710522611052617No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG41430.20537026131544042No Hit
AGTGCTGTCATCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC37670.18673178237394736No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA37160.18420369081539378No Hit
GATGTGAAAGTAACCCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC36870.18276614855660842No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAACCTCGTAT36120.1790483668528532TruSeq Adapter, Index 13 (97% over 38bp)
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA35370.1753305851490979No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC32940.1632849724289309No Hit
AGTGCTGTCATCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC31760.15743566254835595No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC30710.15223076816309858No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGGGAGAAGCGCTCCTT30380.15059494421344627No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA27860.13810319768882862No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC26520.13146076104478588No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC25710.1274455568047302No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCGGACCCCGGTCCCGGCG25690.12734641595929672No Hit
AGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGA25360.12571059200964443No Hit
GCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAA25010.12397562721455863No Hit
GATGCTGGAATCACCCAGAGCCCAAGATACAAGATCACAGAGACAGGAAG23530.11663920465248159No Hit
GAAGCCCAAGTGACCCCAGCAGGGTTACTTTCACATCAGATCGGAAGAGC23510.11654006380704812No Hit
GAACCTGAAGTCACCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC23330.11564779619814686No Hit
GATGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA23280.11539994408456317No Hit
GTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAACC22980.11391283140306106No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCTGGTAGATGTCAGCTTC22030.10920364124497109No Hit
GATGTGAAAGTAACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC21470.10642769757283382No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAAGCTCGTAT20890.10355261305526309TruSeq Adapter, Index 13 (97% over 38bp)
AACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACAG20380.10102452149670953No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)