FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017332
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT1751648.682953524754478Illumina Single End PCR Primer 1 (96% over 32bp)
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC1429977.0884217372252065No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA1345496.669650806114215No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA1262276.257125748265531No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA529192.6232171997469926No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA226031.120440264666401No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA206881.02551290516385No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC205291.017631207951889No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC185800.9210184540769689No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA178830.8864678694434035No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA146230.7248682913868416No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA129530.6420856854498913No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCGGTGCTGTCGTCTCTCT104040.5157306779449293No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATAT94640.46913448059119667Illumina Single End PCR Primer 1 (96% over 32bp)
GATGCCATGGTCATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT81770.4053373465547565No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGAACT73430.363995614008998Illumina Single End PCR Primer 1 (96% over 32bp)
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG68230.33821899419629486No Hit
AAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAG66560.32994073360259984No Hit
GATGTGAAAGTAACCCAGAAGCCCAAGTGACCCAGAACCCAAGATACCTC63770.3161105856646303No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC61120.30297442364469507No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATTT57220.28364195878516774Illumina Single End PCR Primer 1 (96% over 32bp)
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG54170.26852297985656304No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG53080.2631198037804387No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCGGTGCTGTCGTCTCTCT51980.25766705728159767No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC48740.24160624032137498No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG48700.24140795863050798No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA48690.2413583882077913No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA48350.23967299383542223No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG46620.23109731070542677No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG46380.22990762056022512No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA45420.2251488599794184No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCGTCG41970.20804706414214416No Hit
GATGCTGAAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC39430.19545617677209304No Hit
GTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAACC34380.17042311330014098No Hit
GATGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA33020.16368153581066477No Hit
GGTGAAGAAGTCGCCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCA32440.16080645129309407No Hit
GAACCTGAAGTCACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG31810.15768351466193964No Hit
AGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC31420.1557502681759869No Hit
GTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAACCTCTGT30520.1512889301314806No Hit
AGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGA29480.14613360616893997No Hit
GATGCTGGAATCACCCAGAGCCCAAGATACAAGATCACAGAGACAGGAAG29230.14489434560102155No Hit
GCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAAGAA29160.14454735264200438No Hit
ATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACAG28990.14370465545581987No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCGACACAGCCGTTTCCCA28060.13909460614316335No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCGGACCCCGGTCCCGGCG26350.13061806385860136No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG25640.12709856384571305No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGGGAGAAGCGCTCCTT23890.11842373987028412No Hit
GATGTGAAAGTAACCCTGCTGGGGTCACTTGGGCTTCAGATCGGAAGAGC23400.11599478915716402No Hit
AGTGCTGTCATCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG23200.11500338070282928No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA21950.10880707786323719No Hit
GAAGCCCAAGTGACCCCAGCAGGGTTACTTTCACATCAGATCGGAAGAGC21400.10608070461381666No Hit
AACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACAG21020.10419702855058066No Hit
AGTGCTGTCATCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC20850.10335433136439613No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAAATCT20740.10280905671451203Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)