FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015755
Sequences flagged as poor quality0
Sequence length300
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT14967914.735738440864186TruSeq Adapter, Index 7 (97% over 38bp)
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA278412.7409168549502585No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC173621.7092704441523794No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC168061.6545328351817121No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA149791.4746666272870919No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA102051.0046714020605363No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA89400.8801334967585688No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA86570.8522724475882472No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC73970.7282267869712677No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA68260.6720124439456364No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC48960.4820059955402632No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA40500.3987181948402912No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAACTCGTAT39420.3880857096445501TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCACCTCGTAT34370.3383689964607607TruSeq Adapter, Index 7 (97% over 38bp)
GAAGCCCAAGTGACCCAGGCTGGTGACCTTCCTGGACAGCAGCAACAGGC29860.29396852587484185No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC29410.2895383237099497No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA29200.2874708960330001No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA27050.26630437457851547No Hit
GATGGTGGAATCACTCAGTCCCCGAAGTACCTGTTCAGAAAGGAAGGACA25030.24641768930499974No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC24830.24444871056504766No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC23570.23204414450334973TruSeq Adapter, Index 7 (97% over 35bp)
GAAGCCCAAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGC23500.2313550019443665No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA23010.22653100403148396No Hit
GACACAGCCGTTTCCCTTCATTCTTTAAAAGATGACAACTGGAGAGCTTT20680.2035924017110425No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC19960.19650407824721514No Hit
GGTGAAGAAGTCGCCCAGGCGGAACGATACGGCAGCGCCGCGGAGCCTCG19940.19630718037321992No Hit
GGTGAAGAAGTCGCCCAGCTTAGGGACCGGGTTTTCCTGGACTGTGGGGG19530.1922707739563182No Hit
GATGCTGGAATCACCCAGACCTGCATTTCCCACTGGTCCAGTTGCTTCTT19320.19020334627936855No Hit
GATGCCATGGTCATCCACACACTACCATACCTCTTTCGCTATAAAATGGC18790.18498555261849559No Hit
ACTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG17470.17199029293481202No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAGCTCGTAT17080.16815078439190553TruSeq Adapter, Index 7 (97% over 38bp)
GATGCCATGGTCATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT16650.16391748010100862No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC16630.1637205822270134No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC14820.1459013246304473No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA14680.14452303951248086No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA14190.1396990415995983No Hit
GAAGCTGGAGTGGCCCAGTCTCCCAGATATAAGATTATAGAGAAAAGGCA13850.13635177774167984No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC12610.1241441095539771No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC12390.12197823294002982No Hit
GAAGCCCAAGTGACCCAGATGCTGAGGCAGGACTGGCACTGAGGTCCACT12380.12187978400303222No Hit
GGTGAAGAAGTCGCCCAGATGCACCAGAACTTCTCGCAGCAGCTGCTCCA12350.12158443719203942No Hit
AATGCCGGCGTCATGCAGAAGGAGCACAGGTTAGTGTCCACCAGCACAGG11830.11646509246816407No Hit
AGCTGGAGTCTCCCAGTCCCCCAGTAACAAGGTCACAGAGAAGGGAAAGG11690.11508680735019763No Hit
AGTGCTGTCATCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC11540.1136100732952336No Hit
AATGCCGGCGTCATGCAGAAGGAGCACAGGTTAGCGTCCACCAGCACAGG10660.10494656683944456No Hit
AATGCCGGCGTCATGCAGATGACTACTTTCCAGCTGGAGGTTCTGAGACT10570.10406052640646613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)