FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015755
Sequences flagged as poor quality0
Sequence length300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT11587911.408164370345212Illumina Single End PCR Primer 1 (96% over 32bp)
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC347893.4249400692096024No Hit
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA345713.403478200944125No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT241572.37823097105109Illumina Single End PCR Primer 1 (96% over 32bp)
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC181041.7823195554046003No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA156181.53757549802856No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA116991.1517541139349547No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA98360.9683437443084207No Hit
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA95250.9377261249021664No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC94760.9329021269892839No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA81660.8039340195224242No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC57210.5632263685632855No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA55940.5507233535645899No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC51240.5044523531757166No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCGCTATGTGTAGATCT44550.4385900143243204Illumina Single End PCR Primer 1 (96% over 32bp)
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC35710.35156115401843946No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA32790.3228140644151395No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA30490.30017080890569087No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG29930.29465766843382507No Hit
GACACAGCCGTTTCCCTTCATTCTTTAAAAGATGACAACTGGAGAGCTTT29400.28943987477295213No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCACTATGTGTAGATCT27730.2729989022943525Illumina Single End PCR Primer 1 (96% over 32bp)
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA26670.2625633149726066No Hit
GAAGCCCAAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGC26400.2599051936736713No Hit
AGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC24860.2447440573760405No Hit
GATGGTGGAATCACTCAGTCCCCGAAGTACCTGTTCAGAAAGGAAGGACA24000.23627744879424667No Hit
GGTGAAGAAGTCGCCCAGCTTAGGGACCGGGTTTTCCTGGACTGTGGGGG20750.20428154427002573No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCGTCG20540.2022141165930761No Hit
GAAGCCCAAGTGACCCAGGCTGGTGACCTTCCTGGACAGCAGCAACAGGC18350.18065379939060108No Hit
GATGCCATGGTCATCCACACACTACCATACCTCTTTCGCTATAAAATGGC18350.18065379939060108No Hit
GATGCCATGGTCATCCAGAACCCAAGATACCAGGTTACCCAGTTTGGAAA18030.1775034334066778No Hit
GATGCTGGAATCACCCAGACCTGCATTTCCCACTGGTCCAGTTGCTTCTT17210.16943062057287436No Hit
ACTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG17100.16834768226590072No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA15880.1563369119521932No Hit
GGTGAAGAAGTCGCCCAGGCGGAACGATACGGCAGCGCCGCGGAGCCTCG15340.15102066935432262No Hit
GAAGCTGGAGTGGCCCAGTCTCCCAGATATAAGATTATAGAGAAAAGGCA15210.14974083317335382No Hit
GACACAGCCGTTTCCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG15020.14787030337039936No Hit
GGTGAAGAAGTCGCCCAGATGCACCAGAACTTCTCGCAGCAGCTGCTCCA13090.12886965852986204No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCGTCG13050.12847586278187162No Hit
GGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC12610.1241441095539771No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCGTCG12520.12325806912099867No Hit
GAACCTGAAGTCACCCAGAGAGATGACAGCACTAGATCGGAAGAGCGTCG11920.1173511329011425No Hit
ACTGGAGTTACGCAGACACCAAGACACCTGGTCATGGGAATGACAAATAA11790.11607129672017366No Hit
AGCTGGAGTCTCCCAGTCCCCCAGTAACAAGGTCACAGAGAAGGGAAAGG11350.11173954349227913No Hit
AATGCCGGCGTCATGCAGAAGGAGCACAGGTTAGTGTCCACCAGCACAGG11120.10947521794133427No Hit
AATGCCGGCGTCATGCAGAAGGAGCACAGGTTAGCGTCCACCAGCACAGG10600.10435587321745894No Hit
GAAGCCCAAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGC10220.10061481361155003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)