FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005409282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005409282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742348
Sequences flagged as poor quality0
Sequence length300
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTGAAAGTAACCCAGAGCTCGAGATATCTAGTCAAAAGGACGGGAGA590403.3885308790207236No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCAGGGTTATCTGTAAGAGTGGAAC268801.5427457660582158No Hit
AGTGCTGTCATCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC243561.3978837752274516No Hit
GGTGCTGTCGTCTCTCAACATCCGAGCTGGGTTATCTGTAAGAGTGGAAC238841.3707938942163105No Hit
GATGGTGGAATCACTCAGTCCCCAAAGTACCTGTTCAGAAAGGAAGGACA224121.2863101975035987No Hit
GACACAGCCGTTTCCCAGACTCCAAAATACCTGGTCACACAGATGGGAAA170910.9809177041555418No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT155580.8929329846850341TruSeq Adapter, Index 7 (97% over 38bp)
GAAGCCCAAGTGACCCAGAACCCAAGATACCTCATCACAGTGACTGGAAA140570.8067848673169769No Hit
GGTGCTGTCGTCTCTCAAAAGCCAAGCAGGGATATCTGTCAACGTGGAAC140020.8036282074533906No Hit
GAACCTGAAGTCACCCAGACTCCCAGCCATCAGGTCACACAGATGGGACA125530.7204645685018148No Hit
GGTGCTGTCGTCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC121110.6950965019617206No Hit
GATGCTGATGTTACCCAGACCCCAAGGAATAGGATCACAAAGACAGGAAA120320.6905623905212965No Hit
GAACCTGAAGTCACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC117530.6745495159405585No Hit
AATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAGGAGGAGGGGACA115730.6642186291142758No Hit
GGTGCTGTCGTCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC108960.6253630158843124No Hit
GACACAGCCGTTTCCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC99650.5719293734661502No Hit
AGTGCTGTCATCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC72950.418687885542957No Hit
GAAGCCCAAGTGACCCAGTGTGACTCCAGAATCAGATCGGAAGAGCACAC72320.41507207515375805No Hit
GATTCTGGAGTCACACTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC72220.41449813699674226No Hit
GATGCTGGAATCACCCAGAGCCCAAGACACAAGGTCACAGAGACAGGAAC68960.3957877530780304No Hit
GAAGCCCAAGTGACCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC64000.36732042049005137No Hit
GAAGCCCAAGTGACCCAGGCTGGTGACCTTCCTGGACAGCAGCAACAGGC59790.34315762407969014No Hit
GAAGCTGACATCTACCAGACCCCAAGATACCTTGTTATAGGGACAGGAAA57360.3292109268642085No Hit
GATGGTGGAATCACTCAGTCCCCGAAGTACCTGTTCAGAAAGGAAGGACA55540.3187652524065227No Hit
GAAGCCCAAGTGACCCAGCCTGACATCCACATCACTGTCTCTGAAGGAGC52000.29844784164816673No Hit
GACACAGCCGTTTCCCTTCATTCTTTAAAAGATGACAACTGGAGAGCTTT51530.29575033231019293No Hit
GATTCTGGAGTCACACTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC51390.29494681889037094No Hit
GACACAGCCGTTTCCCAGTGTGACTCCAGAATCAGATCGGAAGAGCACAC46320.2658481543296747No Hit
GAAGCCCAAGTGACCCAGAGAGACGACAGCACCAGATCGGAAGAGCACAC45430.26074010473223486No Hit
GGTGCTGTCGTCTCTCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC43310.24857261580350193No Hit
GAAGCTGGAGTTACTCAGTTCCCCAGCCACAGCGTAATAGAGAAGGGCCA40510.23250234740706222No Hit
GAACCTGAAGTCACCCAGAGACGACAGCACCAGATCGGAAGAGCACACGT36650.21034833454625598No Hit
GAACCTGAAGTCACCCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACAC34520.19812345180182145No Hit
GGTGCTGTCGTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACACGT34440.19766430127620888No Hit
AGTGCTGTCATCTCTCTGGGCGACTTCTTCACCAGATCGGAAGAGCACAC33760.1937615218085021No Hit
GAAGCCCAAGTGACCCAGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC33210.19060486194491572No Hit
GATTCTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACA32530.18670208247720893No Hit
GGTGAAGAAGTCGCCCAGAGAGATGACAGCACTAGATCGGAAGAGCACAC31910.18314366590371153No Hit
AATGCCGGCGTCATGCTGAGCTTATAAGATCGGAAGAGCGAGATCGGAAG31670.18176621432687384No Hit
GCTGCTGGAGTCATCCAGAAGCGTGCTGAGCTTATAAGATCGGAAGAGCG27700.15898086949335036No Hit
GCTGCTGGAGTCATCCAGTCCCCAAGACATCTGATCAAAGAAAAGAGGGA27600.15840693133633466No Hit
GAAGCTGGAGTGGCCCAGTCTCCCAGATATAAGATTATAGAGAAAAGGCA27100.15553724055125612No Hit
CGCTCTTCCGATCTTATAAGCTCAGCATGACGCCGGCATTAGATCGGAAG25210.14468980938365927No Hit
GATGCTGATGTTACCCAGCGTGCTGAGCTTATAAGATCGGAAGAGCGAGA23660.13579376794991585No Hit
AGTGCTGTCATCTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACAC23580.1353346174243033No Hit
GAAGCCCAAGTGACCCAGATGGAAGGGCCAGTGACTCTCTCAGAAGAGGC23220.13326844005904676No Hit
GGTGCTGTCGTCTCTGGGAAACGGCTGTGTCAGATCGGAAGAGCACACGT23170.13298147098053892No Hit
AATGCCGGCGTCATGCAGCCTTCAGCCTCCTCTTTGTCCAAAATACTGCT22410.12861954098721953No Hit
GAACCTGAAGTCACCCAAAGCGTGCTGAGCTTATAAGATCGGAAGAGCGA21530.12356888520548134No Hit
AATGCCGGCGTCATGCAGATGACTACTTTCCAGCTGGAGGTTCTGAGACT21240.12190446455013579No Hit
GACACAGCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCC21180.12156010165592637No Hit
GACACAGCCGTTTCCCAGAGACGACAGCACCAGATCGGAAGAGCACACGT21110.12115834494601538No Hit
GAAGCCCAAGTGACCCAGGTGTGACTCCAGAATCAGATCGGAAGAGCACA19900.11421369324612533No Hit
GACACAGCCGTTTCCCAGTGAAGCTGGAATGAAATCAAGATTTGTCTGGA19380.11122921482964368No Hit
AGTGCTGTCATCTCTCTGGGTGACTTCAGGTTCAGATCGGAAGAGCACAC19150.10990915706850755No Hit
ACTGGAGTCACACAAACCCCAAAGCACCTGATCACAGCAACTGGACAGCG18620.10686728483632432No Hit
GATTCTGGAGTCACACCTGGGTCACTTGGGCTTCAGATCGGAAGAGCACA18490.10612116523220388No Hit
GAACCTGAAGTCACCCAGGCTGCCAACCAAGGTGGCTGAATACTTGCTTG18420.1057194085222929No Hit
GATGCCATGGTCATCCACACACTACCATACCTCTTTCGCTATAAAATGGC17900.10273493010581124No Hit
AATGCCGGCGTCATGCAGGTGACCTCACTCGGACGGAAGAATCTTCCCGA17780.10204620431739238No Hit
CGCTCTTCCGATCTTATAAGCTCAGCACGCTTCTGGATGACTCCAGCAGC17510.10049657129345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)